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Association of GSTM1 and GSTT1 Polymorphisms with Chronic Obstructive Pulmonary Disease in a Tunisian Population

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Abstract

GSTM1 and GSTT1 polymorphisms have been proposed in relationship with chronic obstructive pulmonary disease (COPD). We investigated the association between these polymorphisms and COPD (as well as its subtypes emphysema and chronic bronchitis) in 234 COPD patients and 182 healthy controls in the Tunisian population. Genotyping was performed using multiplex PCR. GSTM1-null genotype frequency was significantly higher in COPD patients than in controls (P = 0.02); however, multivariate analysis of cofounding variables showed no independent association with this genotype (P = 0.073). In contrast, the association of the GSTM1-null genotype with emphysema was significant, even after adjustment for risk factors (P = 0.011). There were no significant differences in GSTT1 genotypes between patients and controls. The GSTM1 null allele is likely not an independent risk factor for COPD but is related to emphysema, whereas the GSTT1 gene is not associated with the disease.

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Acknowledgments

The authors thank Dr. A. Kassab and Dr. A. Miled from Biochemistry Laboratory, CHU Farhat Hached, Sousse, Tunisia, for their suggestions and helpful comments.

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Authors and Affiliations

  1. Biochemistry and Molecular Biology Laboratory, Faculty of Pharmacy, Monastir University, 1 Av. Avicenne, 5019, Monastir, Tunisia

    Ramzi Lakhdar, Sabri Denden, Nadia Leban, Houria Daimi, Jemni Ben Chibani & Amel Haj Khelil

  2. Pulmonology Department, CHU Tahar Sfar, 5111, Mahdia, Tunisia

    Jalel Knani

  3. Haematology Laboratory, Faculty of Pharmacy, CHU Fattouma Bourguiba, 5019, Monastir, Tunisia

    Mohsen Hassine

  4. Institute of Human Genetics, Montpellier 2 University, Montpellier, France

    Gérard Lefranc

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  1. Ramzi Lakhdar
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  2. Sabri Denden
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  3. Jalel Knani
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  9. Amel Haj Khelil
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Correspondence to Ramzi Lakhdar.

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Lakhdar, R., Denden, S., Knani, J. et al. Association of GSTM1 and GSTT1 Polymorphisms with Chronic Obstructive Pulmonary Disease in a Tunisian Population. Biochem Genet 48, 647–657 (2010). https://doi.org/10.1007/s10528-010-9346-z

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  • DOI: https://doi.org/10.1007/s10528-010-9346-z

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