Abstract
The aim of the present study was to explore the role of lncRNA ANRIL in the pathogenesis of ischemic stroke (IS) and coronary artery disease (CAD) and to determine the association between ANRIL variants and the genetic susceptibility of IS and CAD in the Chinese Han population. A genetic association study including 550 IS patients, 550 CAD patients, and 550 healthy controls was conducted. The expression levels of lncRNA ANRIL, CDKN2A, and CDKN2B were detected using qRT-PCR. Genotyping was performed by Sequenom MassARRAY on an Agena platform. Our study showed that IS patients had an increased lncRNA ANRIL expression (P = 0.002) and a decreased CDKN2A expression (P < 0.001) compared with normal controls. A significant difference with regard to the genotype distribution of rs2383207 was found between male IS patients and controls (P = 0.011). The minor allele of rs2383207 significantly increased the IS risk under a recessive model (OR = 1.52, 95% CI = 1.05–2.21, P = 0.027). The minor allele of rs1333049 was significantly associated with the risk of IS among the male patients under a recessive model (OR = 1.56, 95% CI = 1.04–2.35, P = 0.031). However, no significant association was found between the ANRIL variants and the risk of CAD (all P > 0.050). In addition, we found a decreased lncRNA ANRIL expression in IS patients who carried the GG genotype of rs1333049 compared with IS patients who carried the CC or CG genotype (P = 0.041). In summary, we found that IS patients had an increased lncRNA ANRIL expression and a decreased CDKN2A expression compared with the controls, which might play an impellent role in pathological processes of IS. The ANRIL variants rs2383207 and rs1333049 were significantly associated with the risk of IS among males but not females in the Chinese Han population.
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Acknowledgements
This study was funded by Grants from National Natural Science Foundation of China (Nos. 81573756 and 81473670).
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Li Su and Jianxiong Long designed the study protocol and conceived the framework of this article. Jialei Yang, Lian Gu, and Xiaojing Guo collected the study samples and their individual information. Jialei Yang, Xiaojing Guo, Jiao Huang, Zhaoxia Chen, Guifeng Huang Yiwen Kang, and Xiaoting Zhang performed the experiments. Zhaoxia Chen and Guifeng Huang conducted the statistical analysis. Then, Jialei Yang, Lian Gu, and Xiaojing Guo wrote the first draft of this manuscript, all of the authors made contributions to the final version of the manuscript.
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Yang, J., Gu, L., Guo, X. et al. LncRNA ANRIL Expression and ANRIL Gene Polymorphisms Contribute to the Risk of Ischemic Stroke in the Chinese Han Population. Cell Mol Neurobiol 38, 1253–1269 (2018). https://doi.org/10.1007/s10571-018-0593-6
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DOI: https://doi.org/10.1007/s10571-018-0593-6