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CTHRC1 promotes osteogenic differentiation of periodontal ligament stem cells by regulating TAZ

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Abstract

Collagen triple helix repeat containing 1 (CTHRC1) is associated with bone metabolism. Alveolar bone has an ability to rapidly remodel itself to adapt its biomechanical environment and function. However, whether CTHRC1 is expressed in alveolar bone tissue and the role of CTHRC1 in alveolar bone remodeling remain unclear. We used orthodontic tooth movement (OTM) rat model to study the effects of CHTRC1 in alveolar bone remodeling in vivo. We found that CTHRC1 was expressed in normal physiological condition of osteocytes, bone matrix, and periodontal ligament cells in rat. During the OTM, the expression of CTHRC1, Runx2 and TAZ were increased. We further studied the effects of CTHRC1 on osteogenic differentiation of human periodontal ligament stem cells in vitro. CTHRC1 can positively regulate the expression of TAZ and osteogenic differentiation markers like Col1, ALP, Runx2 and OCN. Overexpression of CHTRC1 increased osteogenic differentiation of PDLSCs, which could be abolished by TAZ siRNA. Our results suggest that CTHRC1 plays an important role in alveolar bone remodeling and osteogenic differentiation of PDLSCs.

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Acknowledgements

This work was supported by Grants from the National Natural Science Foundation of China (Grant No. 81300885 and 81402150), China Postdoctoral Science Foundation (Grant No: 2017M610432),  Shandong Provincial Key Research and Development Program (Grant No: 2015GSF118122, 2016GSF201115 and 2016GSF201220), Young Scholars Program of Shandong University (Grant No: 2015WLJH53) and the Construction Engineering Special Fund of Taishan Scholars (Grant No: ts201511106).

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    Authors and Affiliations

    1. School of Stomatology, Shandong University, No. 44-1, Wenhua Xi Road, Jinan, 250012, Shandong, People’s Republic of China

      Chengze Wang, Baiyu Sun, Yunpeng Zhang, Yawen Ji, Xin Xu & Yong Wen

    2. Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan, People’s Republic of China

      Chengze Wang, Baiyu Sun, Yunpeng Zhang, Yawen Ji, Xin Xu & Yong Wen

    3. Qilu Hospital of Shandong University, Jinan, People’s Republic of China

      Weiting Gu

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    1. Chengze Wang
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    Correspondence to Xin Xu or Yong Wen.

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    Chengze Wang and Weiting Gu have contributed equally to this work.

    X Xu and Y Wen have contributed equally to this work.

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    Wang, C., Gu, W., Sun, B. et al. CTHRC1 promotes osteogenic differentiation of periodontal ligament stem cells by regulating TAZ. J Mol Hist 48, 311–319 (2017). https://doi.org/10.1007/s10735-017-9729-0

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