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Laser micro-patterning of biodegradable polymer blends for tissue engineering

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Abstract

We propose a multistep all laser, maskless, and solvent free synthesis of micro-patterned substrates of biodegradable polymer blends, with applicability for guided cell adhesion and localized hyaluronic acid (HA) immobilization. The polymer blends comprised polyurethane (PU), poly(lactic-co-glycolic acid) (PLGA), and polylactide-polyethylene glycol-polylactide (PPP) in 1:1:1 blending ratios. Polymer patterning was performed by laser processing in two steps. First, the polymers were patterned with periodic micro-channels by direct femtosecond laser ablation, which provided flexibility in design and spatial accuracy for the patterns. As a second step, the micro-patterned polymers were coated with thin layers of polymer blends using matrix assisted pulsed laser evaporation (MAPLE). The resulted sandwich substrates were composed of a bottom, micro-patterned layer and thin, top layer which conserved the patterns from the underlying layer and preserved the polymers chemical composition. Depending on the bottom/top layers, the substrates were denominated PU/PU:PLGA:PPP and PU:PLGA:PPP/PU:PLGA:PPP, respectively. The laser generated micro-patterns were used for selective attachment of oral keratinocyte stem cells and for HA immobilization. The highest cellular density was found on the PU:PLGA:PPP/PU:PLGA:PPP substrate, where the spongy-like micro-channels provided multiple anchoring points for the cells. For both substrates, the micro-channels enabled localized immobilization of HA. The effectiveness of HA immobilization was tested against cell adhesion and protein adsorption.

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Acknowledgements

The research presented in this paper was financed by the contract UEFIS-CDI, PN-II-PT-PCCA no. 6/2012 from the Romanian Government. BC would like to acknowledge that this paper is partly supported by the Sectorial Operational Programme Human Resources Development (SOPHRD), financed by the European Social Fund and the Romanian Government under the contract number POSDRU 141531.

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Authors and Affiliations

  1. Faculty of Applied Sciences, University Politehnica of Bucharest, 060042, Bucharest, Romania

    Irina Alexandra Paun & Mona Mihailescu

  2. National Institute for Laser, Plasma and Radiation Physics, Magurele, 077125, Bucharest, Romania

    Irina Alexandra Paun, Marian Zamfirescu, Catalin Romeo Luculescu & Maria Dinescu

  3. Horia Hulubei National Institute for Physics and Nuclear Engineering IFIN-HH, Magurele, 077125, Bucharest, Romania

    Cosmin Catalin Mustaciosu & Ion Dorobantu

  4. Department of Biochemistry, Faculty of Dentistry, UMF Carol Davila, Bucharest, Romania

    Bogdan Calenic

  5. Department of Proteomics Biochemistry, Victor Babes National Institute of Pathology, 050096, Bucharest, Romania

    Bogdan Calenic

Authors
  1. Irina Alexandra Paun
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  2. Marian Zamfirescu
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  3. Mona Mihailescu
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  4. Catalin Romeo Luculescu
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  5. Cosmin Catalin Mustaciosu
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  6. Ion Dorobantu
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  7. Bogdan Calenic
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  8. Maria Dinescu
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Correspondence to Irina Alexandra Paun, Catalin Romeo Luculescu or Maria Dinescu.

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Paun, I.A., Zamfirescu, M., Mihailescu, M. et al. Laser micro-patterning of biodegradable polymer blends for tissue engineering. J Mater Sci 50, 923–936 (2015). https://doi.org/10.1007/s10853-014-8652-y

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  • DOI: https://doi.org/10.1007/s10853-014-8652-y

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