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In vitro study in stimulating the secretion of angiogenic growth factors of strontium-doped calcium polyphosphate for bone tissue engineering

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Abstract

Angiogenesis of tissue-engineered bone remains a limited factor for the engineering of larger bone tissue constructs. Attempts to stimulate angiogenesis, using recombinant protein or gene transfer of angiogenic growth factors, have been proposed; however, these approaches have been associated with some problems regarding such as complex technique, expensive prices as well as safety problems and short half-life of angiogenic growth factors. This study was performed to determine the ability of strontium-doped calcium polyphosphate (SCPP) to induce angiogenesis via researching its effect on the mRNA expressions and protein secretion of VEGF and bFGF in/from cultured osteoblasts (ROS17/2.8 cells). We cultured osteoblasts with SCPP scaffolds containing various doses of strontium as well as calcium polyphosphate (CPP) scaffold. Through the detection of MTT and SEM, we have found that SCPP could promote cell proliferation and maintain their morphology. The results of RT–PCR and ELISA indicated that, compared with those in CPP group, the mRNA expression as well as protein levels of VEGF and bFGF in/from cultured osteoblasts were dose-dependent increasing in response to increasing strontium before reaching the peak in SCPP groups, and 8% SCPP showed the optimal promoting role. Therefore, SCPP containing proper dose of strontium could be served as a potential biomaterial with stimulating angiogenesis in bone tissue engineering and bone repair.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (30870616), Basic Research Program of Sichuan Province-China (07JY029-075) and Natural Science Foundation of Jiangsu Province-China (BK2008152). We would like to thank the Analysis and Testing Center, Sichuan University (China) for their assistance of the completion of scanning electron microscopy analysis.

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Authors and Affiliations

  1. College of Polymer Science and Engineering, Sichuan University, 610065, Chengdu, People’s Republic of China

    Fei Liu, Xu Zhang, Xixun Yu, Yuanting Xu & Dawei Ren

  2. Suzhou Institute of Sichuan University, 215123, Suzhou, People’s Republic of China

    Fei Liu & Xixun Yu

  3. West China Second University Hospital, Sichuan University, 610065, Chengdu, People’s Republic of China

    Ting Feng

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  1. Fei Liu
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  2. Xu Zhang
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  3. Xixun Yu
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  4. Yuanting Xu
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Correspondence to Xixun Yu.

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Liu, F., Zhang, X., Yu, X. et al. In vitro study in stimulating the secretion of angiogenic growth factors of strontium-doped calcium polyphosphate for bone tissue engineering. J Mater Sci: Mater Med 22, 683–692 (2011). https://doi.org/10.1007/s10856-011-4247-1

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  • DOI: https://doi.org/10.1007/s10856-011-4247-1

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