Skip to main content

Advertisement

SpringerLink
Log in

Biological monitoring of a xenomaterial for grafting: an evaluation in critical-size calvarial defects

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Our purpose was to evaluate the osteoconduction potential of mixed bovine bone (MBB) xenografts as an alternative for bone grafting of critical-size defects in the calvaria of rats. After surgery, in the time intervals of 1, 3, 6, and 9 months, rats were killed and their skulls collected, radiographed and histologically prepared for analysis. The data obtained from histological analysis reported that the particles of MBB did not promote an intense immunological response, evidencing its biocompatibility in rats. Our results clearly showed the interesting evidence that MBB was not completely reabsorbed at 9 months while a small amount of newly formed bone was deposited by osteoprogenitor cells bordering the defect. However, this discrete bone-forming stimulation was unable to regenerate the bone defect. Overall, our results suggest that the properties of MBB are not suitable for stimulating intense bone regeneration in critical bone defects in rats.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Zambuzzi WF, Oliveira RC, Pereira FL, Cestari TM, Taga R, Granjeiro JM. Rat subcutaneous tissue response to macrogranular porous anorganic bovine bone graft. Braz Dent J. 2006;17(4):274–8.

    Article  Google Scholar 

  2. Molly L, Vandromme H, Quirynen M, Schepers E, Adams JL, Van Steenberghe D. Bone formation following implantation of bone biomaterials into extraction sites. J Periodontol. 2008;79(6):1108–15.

    Article  Google Scholar 

  3. Wenz B, Oesch B, Horst M. Analysis of the risk of transmitting bovine spongiform encephalopathy through bone grafts derived from bovine bone. Biomaterials. 2001;22(12):1599–606.

    Article  CAS  Google Scholar 

  4. Anderson A, Mucalo MR, Johnson GS, Lorier MA. The processing and characterization of animal-derived bone to yield materials with biomedical applications. Part III: material and mechanical properties of fresh and processed bovine cancellous bone. J Mater Sci Mater Med. 2000;11(11):743–9.

    Article  CAS  Google Scholar 

  5. Johnson GS, Mucalo MR, Lorier MA. The processing and characterization of animal-derived bone to yield materials with biomedical applications: part 1: modifiable porous implants from bovine condyle cancellous bone and characterization of bone materials as a function of processing. J Mater Sci Mater Med. 2000;11(7):427–41.

    Article  CAS  Google Scholar 

  6. Meyer S, Floerkemeier T, Windhagen H. Histological osseointegration of Tutobone: first results in human. Arch Orthop Trauma Surg. 2008;128(6):539–44.

    Article  Google Scholar 

  7. Le Geros RZ. Calcium phosphates in oral biology and medicine. Monographs in Oral Science. Karger. New York, USA, 1991.

  8. Sciadini MF, Dawson JM, Johnson KD. Evaluation of bovine-derived bone protein with a natural coral carrier as a bone-graft substitute in a canine segmental defect model. J Orthop Res. 1997;15(6):844–57.

    Article  CAS  Google Scholar 

  9. Jensen SS, Aaboe M, Pinholt EM, Hjorting-Hansen E, Melsen F, Ruyter IE. Tissue reactions and material characteristics of four bone substitutes. Int J Oral Maxillof Impl. 1996;11(1):55–66.

    CAS  Google Scholar 

  10. Cornelini R, Cangini F, Martuscelli G, Wennström J. Deproteinized bovine bone and biodegradable barrier membranes to support healing following immediate placement of transmucosal implants: a short-term controlled clinical trial. Int J Period Rest Dent. 2004;24(6):555–63.

    Google Scholar 

  11. Tadic D, Epple M. A thorough physicochemical characterisation of 14 calcium phosphate-based bone substitution materials in comparison to natural bone. Biomaterials. 2004;25(6):987–96.

    Article  CAS  Google Scholar 

  12. de Oliveira RC, Carneiro E, Cestari TM, Taga R, Granjeiro JM. Dynamics of subcutaneous tissue response to the implantation of tetracycline-treated or untreated membrane of demineralized bovine cortical bone in rats. J Biomater Appl. 2006;21(2):167–78.

    Article  Google Scholar 

  13. Luna LG. Manual of histologic staining methods of the armed forces institute of pathology. New York, USA: McGraw-Hill Book Company; 1968.

    Google Scholar 

  14. Frame JW. A convenient animal model for testing bone substitute materials. J Oral Surg. 1980;38(3):176–80.

    CAS  Google Scholar 

  15. Selvig KA. Discussion: animal models in reconstructive therapy. J Periodontol. 1994;65(12):1169–72.

    CAS  Google Scholar 

  16. Wang HL, Caroll WJ. Guided bone regeneration using bone grafts and collagen membranes. Quint Int. 2001;3(7):504–15.

    Google Scholar 

  17. Bosch C, Melsen B, Vargervik K. Importance of the critical-size bone defect in testing bone-regenerating materials. J Craniofac Surg. 1998;9(4):310–6.

    Article  CAS  Google Scholar 

  18. Taga ML, Granjeiro JM, Cestari TM, Taga R. Healing of critical-size cranial defects in guinea pigs using a bovine bone-derived resorbable membrane. Int J Oral Maxillofac Impl. 2008;23(3):427–36.

    Google Scholar 

  19. Herculano MA, Tella OI Jr, Prandini MN, Alves MTS. Inhibition of peridural fibrosis after laminectomy using biological sheet in rat model. Arq Neuropsiquiatr. 2006;64(2A):259–63.

    Article  Google Scholar 

  20. Zambuzzi WF, Paiva KB, Menezes R, Oliveira RC, Taga R, Granjeiro JM. MMP-9 and CD68(+) cells are required for tissue remodeling in response to natural hydroxyapatite. J Mol Histol. 2009;40(4):301–9.

    Article  CAS  Google Scholar 

  21. Bertazzo S, Zambuzzi WF, Campos DD, Ferreira CV, Bertran CA. A simple method for enhancing cell adhesion to hydroxyapatite surface. Clin Oral Implants Res. 2010;21(12):1411–3.

    Article  Google Scholar 

  22. Bertazzo S, Zambuzzi WF, Campos DD, Ogeda TL, Ferreira CV, Bertran CA. Hydroxyapatite surface solubility and effect on cell adhesion. Colloids Surf B Biointerfaces. 2010;78(2):177–84.

    Article  CAS  Google Scholar 

  23. Bertazzo S, Zambuzzi WF, da Silva HA, Ferreira CV, Bertran CA. Bioactivation of alumina by surface modification: a possibility for improving the applicability of alumina in bone, oral repair. Clin Oral Implants Res. 2009;20(3):288–93.

    Article  CAS  Google Scholar 

  24. Zambuzzi WF, Bruni-Cardoso A, Granjeiro JM, Peppelenbosch MP, de Carvalho HF, Aoyama H, Ferreira CV. On the road to understanding of the osteoblast adhesion: cytoskeleton organization is rearranged by distinct signaling pathways. J Cell Biochem. 2009;108(1):134–44.

    Article  CAS  Google Scholar 

  25. Zambuzzi WF, Milani R, Teti A. Expanding the role of Src and protein-tyrosine phosphatases balance in modulating osteoblast metabolism: lessons from mice. Biochimie. 2010;92(4):327–32.

    Article  CAS  Google Scholar 

  26. Milani R, Ferreira CV, Granjeiro JM, Paredes-Gamero EJ, Silva RA, Justo GZ, Nader HB, Galembeck E, Peppelenbosch MP, Aoyama H, Zambuzzi WF. Phosphoproteome reveals an atlas of protein signaling networks during osteoblast adhesion. J Cell Biochem. 2010;109(5):957–66.

    CAS  Google Scholar 

  27. Laquerriere P, Grandjean-Laquerriere A, Addadi-Rebbah S, Jallot E, Laurent-Maquin D, Frayssinet P, Guenounou M. MMP-2, MMP-9 and their inhibitors TIMP-2 and TIMP-1 production by human monocytes in vitro in the presence of different forms of hydroxyapatite particles. Biomaterials. 2004;25(13):2515–24.

    Article  CAS  Google Scholar 

  28. Laquerriere P, Grandjean-Laquerriere A, Jallot E, Balossier G, Frayssinet P, Guenounou M. Importance of hydroxyapatite particles characteristics on cytokines production by human monocytes in vitro. Biomaterials. 2003;24(16):2739–47.

    Article  CAS  Google Scholar 

  29. Nadra I, Boccaccini AR, Philippidis P, Whelan LC, McCarthy GM, Haskard DO, Landis RC. Effect of particle size on hydroxyapatite crystal-induced tumor necrosis factor alpha secretion by macrophages. Atherosclerosis. 2008;196(1):98–105.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to thank Dr. Flávio Monteiro Amado and Miss Daniele Santi Ceolin for exceptional technical support. This work was supported by CAPES (CAPES Foundation), FAPESP (The State of São Paulo Research Foundation—99/10655-5, 01/10707-7, and 04/08095-1), CNPq (National Council for Scientific and Technological Development—350084/03-3, 475721/2003-9, 505350/04-1 and 504.808/04-4).

Author information

Authors and Affiliations

  1. Department of Endodontics, University of Grande Rio, Duque de Caxias, RJ, 25071-202, Brazil

    Thais Accorsi-Mendonça

  2. Department of Endodontics, Bauru Dental School, University of Sao Paulo, Sao Paulo, Brazil

    Clóvis Monteiro Bramante

  3. Projects Division, The National Institute of Metrology, Standardization and Industrial Quality (INMETRO), Rio de Janeiro, Brazil

    Willian Fernando Zambuzzi & José Mauro Granjeiro

  4. Department of Biological Sciences, Bauru Dental School, University of Sao Paulo, Sao Paulo, Brazil

    Tânia Mari Cestari & Rumio Taga

  5. Metallurgy and Materials Engineering, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil

    Márcia Sader & Glória Dulce de Almeida Soares

  6. Department of Cell and Molecular Biology, Institute of Biology, Fluminense Federal University, UFF, Outeiro de São João Baptista, s/n, Campus do Valonguinho, Centro/Niteroi, RJ, 24020-150, Brazil

    José Mauro Granjeiro

  7. Cell therapy center, University Hospital Antonio Pedro, UFF, Niterói, RJ, Brazil

    Willian Fernando Zambuzzi & José Mauro Granjeiro

Authors
  1. Thais Accorsi-Mendonça
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Willian Fernando Zambuzzi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Clóvis Monteiro Bramante
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tânia Mari Cestari
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rumio Taga
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Márcia Sader
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Glória Dulce de Almeida Soares
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. José Mauro Granjeiro
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to José Mauro Granjeiro.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Accorsi-Mendonça, T., Zambuzzi, W.F., Bramante, C.M. et al. Biological monitoring of a xenomaterial for grafting: an evaluation in critical-size calvarial defects. J Mater Sci: Mater Med 22, 997–1004 (2011). https://doi.org/10.1007/s10856-011-4278-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-011-4278-7

Keywords

Search

Navigation