Abstract
An appropriate cell source, effective cell modification, and proper supportive matrices are the main bases of tissue engineering. The effectiveness of anti-mir221 or hydroxyapatite (HA) in improving the osteogenic differentiation of mesenchymal stem cells (MSCs) has been reported previously. Herein, simultaneous application of these osteogenic inducers was investigated in vivo. The Poly-caprolactone (PCL)/HA nanofibers were characterized using contact angle measurement, tensile test, Fourier transform infrared spectroscopy, and electron microscopy. Rat MSCs were isolated, characterized and transfected with anti-mir221. The rats were divided into 4 groups and an 8 mm defect were created in the mid-calvaria of each rat by trephine bur. Group 1 received (PCL)/HA nanofibers, group 2 received (PCL)/HA nanofibers plus autologous MSCs, group 3 received (PCL)/HA nanofibers plus MSCs transfected with anti-mir221, and group 4 rats were left empty as an additional control group. Histomorphometric and radiomorphometric evaluation after 4 and 8 weeks revealed more new bone formation in the cell-treated groups compared to the scaffold alone group. There was evidence for a combination of increased osteoclasts and osteoblast vascular lake containing red blood cells in the anti-mir221 transfected group. New bone penetration into the scaffolds empirically demonstrated the capability of this combination for efficient osteointegration. Altogether, the co-application of HA and anti-mir221 transfected cells can enhance bone healing of the rat skull.
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Iran national science foundation financially supported this work.
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10856_2016_5746_MOESM2_ESM.tif
Supplementary material 2 (TIFF 624 kb) Supplementary Fig. 1: SEM micrographs of electrospun scaffolds: (A) random fabrication, (B) aligned fabrication, (C) pure PCL, and (D) PCL fibers with nHA incorporation
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Supplementary material 3 (TIFF 1501 kb) Supplementary Fig. 2: Creation of critical-size defect and scaffold implantation in rat calvaria
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Sadeghi, M., Bakhshandeh, B., Dehghan, M.M. et al. Functional synergy of anti-mir221 and nanohydroxyapatite scaffold in bone tissue engineering of rat skull. J Mater Sci: Mater Med 27, 132 (2016). https://doi.org/10.1007/s10856-016-5746-x
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DOI: https://doi.org/10.1007/s10856-016-5746-x