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Using Immunoinformatics and Structural Approaches to Design a Novel HHV8 Vaccine

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International Journal of Peptide Research and Therapeutics Aims and scope Submit manuscript

Abstract

Kaposi’s sarcoma (KS)-associated herpesvirus (KSHV) or human herpesvirus 8 (HHV8) has caused infection in different parts of the world, especially in Africa, the Middle East, and the Mediterranean. HHV8 is a gamma-2 class herpesvirus and its genome contains 26 open reading frames. K1 is a highly variable glycoprotein and can be used as a vaccine candidate. The aim of this study was to design a new vaccine by using immunoinformatics analysis and adding approved adjuvants. In this study, several computational programs were employed to define the most capable region in K1-based immunologic properties; also, different possible adjuvants and universal T-helper agonists were added to the new construct. The construct was examined to define the 3D structure, physicochemical properties and immunologic features. In addition, docking analysis was done to define the ability of the new construct to attach to mTLR. A region (155–170) was selected to be used in the new proposed vaccine; in addition, two TLR agonist adjuvants and two universal T-helpers were added to boost the immune response. Analysis of the new construct showed that the designed protein was able to be used as a vaccine and be expressed in the host. Moreover, the high energy value in docking analysis showed the high ability of this vaccine to induce immune system. The designed vaccine is expected to be capable of generating humoral and cellular responses that are crucial to protect against HHV8 as well as the vaccine has appropriate physicochemical properties and acceptable stability in different host cells.

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Acknowledgements

This study was supported by Grant no. 16304 from the Research Council of Shiraz University of Medical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran. The authors would like to thank Shiraz University of Medical Sciences, Shiraz, Iran and also Center for Development of Clinical Research of Nemazee Hospital and Dr. Nasrin Shokrpour for editorial assistance. We thank Dr.Hassan Joulaei, Mr.Javad Moayedi for their scientific comments and support.‏‏

Funding

This study was funded by Shiraz University of Medical Sciences (Grant No. 16304).

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Authors and Affiliations

  1. Shiraz HIV/AIDS Research Center, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran

    Behzad Dehghani, Tayebeh Hashempour & Zahra Hasanshahi

Authors
  1. Behzad Dehghani
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  2. Tayebeh Hashempour
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  3. Zahra Hasanshahi
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Correspondence to Tayebeh Hashempour.

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Author Behzad Dehghani declares that he has no conflict of interest. Author Tayebeh Hashempoor declares that she has no conflict of interest. Hassan Joulaei declares that he has no conflict of interest Author Zahra Hasanshahi declares that she has no conflict of interest.

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Dehghani, B., Hashempour, T. & Hasanshahi, Z. Using Immunoinformatics and Structural Approaches to Design a Novel HHV8 Vaccine. Int J Pept Res Ther 26, 321–331 (2020). https://doi.org/10.1007/s10989-019-09839-x

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  • DOI: https://doi.org/10.1007/s10989-019-09839-x

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