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The interaction between ubiquitin C-terminal hydrolase 37 and glucose-regulated protein 78 in hepatocellular carcinoma

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Abstract

The ubiquitin C-terminal hydrolase (UCH) is a subfamily of deubiquitinating enzymes, which consists of four members: UCH-L1, UCH-L3, UCH37, and BRCA1-associated protein-1. Although there is growing evidence that UCH enzymes and human malignancies are closely correlated, there have been few studies on UCH37, especially on its interactions with other proteins. In the current study, a functional proteomic analysis was performed to screen UCH37-interacting proteins in hepatocellular carcinoma (HCC), and glucose-regulated protein 78 was identified as one interacting with UCH37, which was confirmed by co-immunoprecipitation and confocal laser scanning microscopy analysis, suggesting that their interaction could provide a new insight into the mechanism of HCC.

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Abbreviations

2D:

Two-dimensional

co-IP:

Co-immunoprecipitation

DUBs:

Deubiquitinating enzymes

FBS:

Fetal bovine serum

FITC:

Fluorescein isothiocyanate

GRP78:

Glucose-regulated protein 78

HCC:

Hepatocellular carcinoma

hINO80:

Human Ino80 chromatin-remodeling complex

HSPs:

Heat shock proteins

IPTG:

Isopropyl β-d-thiogalactoside

PBS:

Phosphate-buffered saline

SD:

Standard deviation

Ub:

Ubiquitin

UCH:

Ubiquitin C-terminal hydrolase

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Acknowledgments

The authors would like to thank the members of Prof. Xizhong Shen’s laboratory for their helpful discussion and critical reading of the manuscript, and the members of the Key Laboratory of Molecular Medicine (under the auspice of the Ministry of Education), Shanghai Medical School of Fudan University for their technical assistance, and Institutes of Biomedical Sciences, Fudan University for their confocal laser scanning microscopy. The study was partly funded by National Basic Research Program of China (2007CB936000), Major National Science and Technology Projects (2009ZX10004-301, 2008ZX10002-017), Shanghai Science and Technology Commission (10410709400, 10411950100), Shanghai Talent Development Fund (2009-035), National Nature Science Foundation of China (30872503, 81000968), China Postdoctoral Science Foundation Funded Project (20100480542), Innovation Foundation for Graduates of Fudan University (EYE152048) ,and the National Clinical Key Special Subject of China.

Conflict of interest

The authors have no financial conflicts of interest.

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Authors and Affiliations

  1. The Department of Gastroenterology of Zhongshan Hospital, Fudan University, 180 Fenglin Rd, Shanghai, 200032, People’s Republic of China

    Ying Fang, Yushui Ma, Da Fu & Xizhong Shen

  2. The Key Laboratory of Molecular Medicine (Under the Auspice of the Ministry of Education), Shanghai Medical School, Fudan University, Shanghai, 200032, People’s Republic of China

    Jingui Mu & Duan Ma

Authors
  1. Ying Fang
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  2. Jingui Mu
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  3. Yushui Ma
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  4. Duan Ma
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  5. Da Fu
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  6. Xizhong Shen
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Corresponding authors

Correspondence to Da Fu or Xizhong Shen.

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Fang, Y., Mu, J., Ma, Y. et al. The interaction between ubiquitin C-terminal hydrolase 37 and glucose-regulated protein 78 in hepatocellular carcinoma. Mol Cell Biochem 359, 59–66 (2012). https://doi.org/10.1007/s11010-011-0999-7

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  • DOI: https://doi.org/10.1007/s11010-011-0999-7

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