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miR-30a downregulation aggravates pressure overload-induced cardiomyocyte hypertrophy

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Abstract

miRNAs play an important role in the pathogenesis of cardiac hypertrophy and dysfunction. However, little is known about how miR-30a regulates cardiomyocyte hypertrophy. In the study, Male C57BL/6 mice were subjected to thoracic aortic constriction, and hearts were harvested at 3 weeks. We assayed miR-30a expression level by real-time PCR and defined the molecular mechanisms of miR-30a-mediated cardiomyocyte hypertrophy. We found that myocardial expression of miR-30a was decreased in mouse models of hypertrophy and in H9c2 cells treated with phenylephrine. MiR-30a inhibition markedly increased mRNA expression of cardiac hypertrophy markers such as atrial natriuretic factor and brain natriuretic peptide in H9c2, and cell size was increased after miR-30a inhibitor treatment. Downregulated miR-30a activated autophagy by inhibiting beclin-1 expression in H9c2 cell. More important, autophagy inhibition suppressed miR-30a inhibitor-induced cardiomyocyte hypertrophy. Together, our data demonstrated that downregulated miR-30a aggravates pressure overload-induced cardiomyocyte hypertrophy by activating autophagy, thus offering a new target for the therapy of cardiomyocyte hypertrophy.

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Abbreviations

miR-30a:

microRNA-30a

TAC:

Thoracic aortic constriction

PE:

Phenylephrine

ANF:

Atrial natriuretic factor

BNP:

Brain natriuretic peptide

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Authors and Affiliations

  1. Department of Emergency Medicine, The Forth Affiliated Hospital, Harbin Medical University, 37 Yiyuan Street, Harbin, 150001, People’s Republic of China

    Xuesong Yin, Chenghai Peng, Wenhu Ning, Chunyan Li, Zhongqiao Ren, Jihong Zhang, Han Gao & Kan Zhao

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  1. Xuesong Yin
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  2. Chenghai Peng
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  3. Wenhu Ning
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  4. Chunyan Li
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  5. Zhongqiao Ren
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  7. Han Gao
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  8. Kan Zhao
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Correspondence to Kan Zhao.

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Yin, X., Peng, C., Ning, W. et al. miR-30a downregulation aggravates pressure overload-induced cardiomyocyte hypertrophy. Mol Cell Biochem 379, 1–6 (2013). https://doi.org/10.1007/s11010-012-1552-z

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  • DOI: https://doi.org/10.1007/s11010-012-1552-z

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