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The effects of endoplasmic reticulum stress response on duck decorin stimulate myotube hypertrophy in myoblasts

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Abstract

Decorin inhibits the transforming growth factor superfamily to stimulate skeletal muscle hypertrophy. The endoplasmic reticulum (ER) plays a crucial role in the synthesis and folding of proteins. The disruption of ER homeostasis affects protein folding and causes ER stress, which is associated with protein synthesis in muscle cells. The purpose of this study was to establish the mechanism by which decorin promotes myoblast proliferation and myotube hypertrophy as mediated by an ER stress-related pathway. Duck myoblasts were incubated for 24 h with tunicamycin. Our results show that tunicamycin triggered ER stress in myoblasts and led to an increase in protein synthesis via a decorin-dependent pathway. We then transfected the decorin gene into duck myoblasts in vitro, and the results demonstrated that overexpression of duck decorin increased myogenic determination factor and follistatin expression and decreased myogenin and myostatin expression. Moreover, the results demonstrated that overexpression of duck decorin led to higher expression of ER stress marker genes. This increased expression trend can be alleviated in vitro by 4-PBA, which is a chemical chaperone that inhibits palmitate-induced ER stress. Collectively, our data show that duck decorin promotes myoblast proliferation mediated by an ER stress-related pathway.

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Acknowledgments

This work was supported by the National High Technology Research and Development Program of China (No. 2010AA10A109), the National Waterfowl Industrial Technology System (No. CARS-43-6), and the Program for Technology Innovative Research Team of Sichuan Province of China (2011JTD0032).

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Authors and Affiliations

  1. College of Animal Science and Technology, Sichuan Agricultural University, Ya’an, Sichuan, 625014, China

    Lingli Sun, Kai Lu, Hehe Liu, Haohan Wang, Xinxin Li, Chao Yang, Liang Li & Jiwen Wang

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  1. Lingli Sun
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  2. Kai Lu
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  8. Jiwen Wang
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Correspondence to Jiwen Wang.

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Lingli Sun and Kai Lu contributed equally to this work.

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Sun, L., Lu, K., Liu, H. et al. The effects of endoplasmic reticulum stress response on duck decorin stimulate myotube hypertrophy in myoblasts. Mol Cell Biochem 377, 151–161 (2013). https://doi.org/10.1007/s11010-013-1581-2

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