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A cyclophilin A inducible expressed in gonad of zhikong scallop Chlamys farreri

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Abstract

Cyclophilin A (CypA), a receptor for the immunosuppressive agent cyclosporin A (CsA), is a cis–trans peptidyl-prolyl isomerase (PPIase) which accelerates the cis–trans isomerization of prolyl-peptide bonds, interacts with a variety of proteins and therefore regulates their activities. One CypA (designated CfCypA) cDNA was cloned from Chlamys farreri by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) techniques. The full-length cDNA of CfCypA consisted of 1,248 nucleotides with a canonical polyadenylation signal sequence AATAAA, a poly (A) tail, and an open reading frame (ORF) of 495 nucleotides encoding a polypeptide of 164 amino acids. The deduced amino acid sequence shared high similarity with CypA from the other species, indicating that CfCypA should be a new member of the CypA family. Quantitative real-time (RT) PCR was employed to assess the mRNA expression of CfCypA in various tissues and its temporal expression in haemocytes and gonad of scallops challenged with Vibrio anguillarum. The mRNA transcripts of CfCypA could be detected in all the examined tissues with highest expression level in gonad. After bacterial challenge, the expression level of CfCypA was almost unchanged in haemocytes, but up-regulated in gonad and increased to the peak (22.59-fold; P < 0.05) at 4 h post-injection, and then dropped to the original level at 8 h post-injection. These results indicated that CfCypA was constitutive expressed in haemocytes, but could be induced in gonad, and perhaps played a critical role in response to the bacterial challenge in gonad.

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Acknowledgments

The authors would like to thank Dr. Jianguo Su and other laboratory members for technical advice and helpful discussion. This research was supported by 863 High Technology Project (No. 2006AA10A402) from the Chinese Ministry of Science and Technology, China, and grants (No. 30730070) from NSFC to Dr. Linsheng Song.

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  1. College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China

    Xiaoyan Song & Xiaolin Liu

  2. Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd., Qingdao, Shandong, 266071, China

    Xiaoyan Song, Lingling Wang, Linsheng Song, Jianmin Zhao, Huan Zhang, Peilin Zheng, Limei Qiu & Longtao Wu

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  1. Xiaoyan Song
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Correspondence to Linsheng Song.

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Song, X., Wang, L., Song, L. et al. A cyclophilin A inducible expressed in gonad of zhikong scallop Chlamys farreri . Mol Biol Rep 36, 1637–1645 (2009). https://doi.org/10.1007/s11033-008-9363-8

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