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Vitamin D status and vitamin D receptor gene polymorphism in Saudi children with acute lower respiratory tract infection

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A Correction to this article was published on 15 May 2019

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Abstract

There is a significant association exists between vitamin D deficiencies, low respiratory tract infections, and certain types of VDR gene polymorphism. Various studies are being conducted to prove any such link between the different clinical conditions due to disturbed vitamin D regulation and VDR gene polymorphisms. The present study analyzed the presence of vitamin D receptor (VDR) gene polymorphisms (ApaI and TaqI) in Saudi pediatric patient suffering from acute lower respiratory tract infection (ALRTI) cases. Fifty children (50) with ALRTI admitted at King Saud University Medical City, Riyadh/Saudi Arabia were included in addition to seventy-three (73) apparently healthy children who were considered as the control group. Genomic DNA from whole blood was extracted and subjected to polymerase chain reaction (PCR) targeting TaqI and ApaI VDR polymorphisms. RFLP–PCR genotyping was performed to determine the allelic frequency within the VDR gene. In the whole sample, the allelic frequency of ApaI polymorphism in the VDR gene was 58.5%, 17.9%, and 23.6% for AA, Aa, and aa respectively (p = 0.11), while it was 48%, 19%, and 33% for TT, Tt, and tt respectively (p = 0.33) with regards to the frequency of TaqI polymorphism in the VDR gene. VDR ApaI Aa and aa genotypes and VDR TaqI Tt and tt genotypes were not associated with increased risk of ALRTI in children (OR 0.87, 95% CI 0.33–2.28, p = 0.77; OR 0.56, 95% CI 0.23–1.4, p = 0.21; OR 1.15, 95% CI 0.44–2.99, p = 0.77; OR 0.73, 95% CI 0.32–1.68, p = 0.46 respectively). To conclude, neither vitamin D status nor VDR gene polymorphisms such as ApaI and TaqI is associated with increased susceptibility to ALRTI. Linkage disequilibrium was not detected between ApaI and TaqI VDR gene polymorphisms as in the case of serum vitamin D status in ALRTI patients versus apparent healthy children.

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  • 15 May 2019

    The correct affiliations for all authors are as given below.

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Acknowledgements

The authors would like to thank the Deanship of Scientific Research, and Research Center, College of pharmacy King Saud University, Riyadh, Saudi Arabia for assisting this study.

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Authors and Affiliations

  1. Clinical Pharmacy Department, College of Pharmacy, King Saud University, PO Box 2454, Riyadh, 11451, Kingdom of Saudi Arabia

    Wael Mansy

  2. Medical Microbiology and Immunology Department, College of Medicine, Beni Suef University, Beni Suef, Egypt

    Nermin H. Ibrahim

  3. Clinical Pathology Department, College of Medicine, Fayoum University, Faiyum, Egypt

    Somaya AL-Gawhary

  4. Pediatric Infectious Diseases unit, King Saud University Medical City, King Saud University, Riyadh, Kingdom of Saudi Arabia

    Sarah S. Alsubaie

  5. Pediatric Clinical Pharmacy Services, King Saud University Medical City, King Saud, University, Riyadh, Kingdom of Saudi Arabia

    Manal M. Abouelkheir

  6. Pharmacology and Toxicology Department, College of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia

    Amal Fatani

  7. Pediatrics Department, College of Medicine, Fayoum University, Faiyum, Egypt

    Fadwa Abd Al Reheem

  8. Pharmaceutics Department, College of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia

    Heba El Awady

  9. Pharmacology Department, Faculty of Medicine, Cairo University, Cairo, Egypt

    Wael Mansy & Enas A. Zakaria

Authors
  1. Wael Mansy
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  2. Nermin H. Ibrahim
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  3. Somaya AL-Gawhary
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  4. Sarah S. Alsubaie
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  5. Manal M. Abouelkheir
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  6. Amal Fatani
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  7. Fadwa Abd Al Reheem
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  8. Heba El Awady
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  9. Enas A. Zakaria
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Contributions

Somaya Al Gowhary and Nermin Hassan prepared the plan for the study and supervised the design and execution. Enas Zakaria, Amal Fatani provided the materials and kits required in the lab. Sarah and Manal facilitated the hospital communication and provided clinical data. Enas Zakaria also collected the samples and performed the laboratory work. Wael Mansy wrote the paper, Fadwa Abdel Reheem and Heba Al Awady reviewed it.

Corresponding author

Correspondence to Wael Mansy.

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Mansy, W., Ibrahim, N.H., AL-Gawhary, S. et al. Vitamin D status and vitamin D receptor gene polymorphism in Saudi children with acute lower respiratory tract infection. Mol Biol Rep 46, 1955–1962 (2019). https://doi.org/10.1007/s11033-019-04645-6

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  • DOI: https://doi.org/10.1007/s11033-019-04645-6

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