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Evaluation of virus resistance and agronomic performance of rice cultivar ASD 16 after transfer of transgene against Rice tungro bacilliform virus by backcross breeding

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Abstract

Severe losses of rice yield in south and southeast Asia are caused by Rice tungro disease (RTD) induced by mixed infection of Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV). In order to develop transgene-based resistance against RTBV, one of its genes, ORF IV, was used to generate transgenic resistance based on RNA-interference in the easily transformed rice variety Pusa Basmati-1, and the transgene was subsequently introgressed to rice variety ASD 16, a variety popular in southern India, using transgene marker-assisted selection. Here, we report the evaluation of BC3F4 and BC3F5 generation rice plants for resistance to RTBV as well as for agronomic traits under glasshouse conditions. The BC3F4 and BC3F5 generation rice plants tested showed variable levels of resistance, which was manifested by an average of twofold amelioration in height reduction, 1.5-fold decrease in the reduction in chlorophyll content, and 100- to 10,000-fold reduction in the titers of RTBV, but no reduction of RTSV titers, in three backcrossed lines when compared with the ASD 16 parent. Agronomic traits of some of the backcrossed lines recorded substantial improvements when compared with the ASD 16 parental line after inoculation by RTBV and RTSV. This work represents an important step in transferring RTD resistance to a susceptible popular rice variety, hence enhancing its yield in areas threatened by the disease.

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References

  1. D. Nagadhara, S. Ramesh, I.C. Pasalu, Y.K. Rao, N.V. Krishnaiah, N.P. Sarma, D.P. Bown, J.A. Gatehouse, V.D. Reddy, K.V. Rao, Plant Biotechnol. J. 1, 231–240 (2003)

    Article  CAS  PubMed  Google Scholar 

  2. S. Dai, R.N. Beachy, In Vitro Cell Dev. B 45, 517–524 (2009)

    Article  CAS  Google Scholar 

  3. K. Muralidharan, D. Krishnaveni, N.V.L. Rajarajeshwari, A.S.R. Prasad, Curr. Sci. 85, 1143–1147 (2003)

    Google Scholar 

  4. H. Hibino, M. Roechan, S. Sudarisman, Phytopathology 68, 1412–1416 (1978)

    Article  Google Scholar 

  5. M.C. Jones, K. Gough, I. Dasgupta, B.L. Subba Rao, J. Cliffe, R. Qu, P. Shen, M. Kaniewska, M. Blakebrough, J.W. Davies, R.N. Beachy, R. Hull, J. Gen. Virol. 72, 757–761 (1991)

    Article  CAS  PubMed  Google Scholar 

  6. J.M. Hay, M.C. Jones, M.L. Blakebrough, I. Dasgupta, J.W. Davies, R. Hull, Nucleic Acids Res. 19, 2615–2621 (1991)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. P. Shen, M. Kaniewska, C. Smith, R.N. Beachy, Virology 193, 621–630 (1993)

    Article  CAS  PubMed  Google Scholar 

  8. H. Sanfacon, J. Wellink, O. Le Gall, A. Karasev, R. van der Vluqt, T. Wetzel, Arch. Virol. 154, 899–907 (2009)

    Article  CAS  PubMed  Google Scholar 

  9. E. Jacquot, M. Keller, P. Yot, Virology 239, 352–359 (1997)

    Article  CAS  PubMed  Google Scholar 

  10. D. Leclerc, L. Burri, A.V. Kajava, J.L. Mougeot, D. Hess, A. Lustig, G. Kleemann, T. Hohn, J. Biol. Chem. 273, 29015–29021 (1998)

    Article  CAS  PubMed  Google Scholar 

  11. E. Herzog, O. Guerra-Peraza, T. Hohn, J. Virol. 74, 2073–2083 (2000)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. R.D. Qu, M. Bhattacharya, G.S. Laco, A. De Kochko, B.L. Rao, M.B. Kaniewska, J.S. Elmer, D.E. Rochester, C.E. Smith, R.N. Beachy, Virology 185, 354–364 (1991)

    Article  CAS  PubMed  Google Scholar 

  13. H. Kano, M. Koizumi, H. Noda, H. Hibino, K. Ishikawa, T. Omura, P.Q. Cabauatan, H. Koganezawa, Arch. Virol. 124, 157–163 (1992)

    Article  CAS  PubMed  Google Scholar 

  14. G.S. Laco, R.N. Beachy, Proc. Natl Acad. Sci. USA 91, 2654–2658 (1994)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. R. Hull, Annu. Rev. Phytopathol. 34, 275–297 (1996)

    Article  CAS  PubMed  Google Scholar 

  16. R. Rajeswaran, V. Golyaev, J. Sequin, A.S. Zvereva, L. Farinelli, M.M. Pooggin, Mol. Plant Microbe Interact. 27, 1370–1378 (2014)

    Article  PubMed  Google Scholar 

  17. I. Dasgupta, R. Hull, S. Eastop, C. Poggi-pollini, M. Blakebrough, M.I. Boulton, J.W. Davies et al., J. Gen. Virol. 72, 1215–1221 (1991)

    Article  CAS  PubMed  Google Scholar 

  18. C.T. Rivera, S.H. Ou, Plant Dis. Rep. 51, 877–881 (1967)

    Google Scholar 

  19. P.Q. Cabauatan, H. Hibino, Phytopathology 21, 103–109 (1985)

    Google Scholar 

  20. O. Azzam, T.C.B. Chancellor, Plant Dis. 86, 88–100 (2002)

    Article  CAS  Google Scholar 

  21. T.W. Mew, H. Leung, S. Savary, C.M.V. Cruz, J.E. Leach, Crit. Rev. Plant Sci. 23, 103–127 (2004)

    Article  Google Scholar 

  22. I.S. Manwan, G. Sama, S.A. Rizvi, Indones. Agric. Res. Dev. J. 7, 43–48 (1985)

    Google Scholar 

  23. G. Dahal, H. Hibino, R.C. Saxena, Phytoplathology 80, 371–377 (1990)

    Article  Google Scholar 

  24. H. Tyagi, S. Rajasubramaniam, M.V. Rajam, I. Dasgupta, Transgenic Res. 17, 897–904 (2008)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. U. Ganesan, S.S. Suri, S. Rajasubramaniam, M.V. Rajam, I. Dasgupta, Virus Genes 39, 113–119 (2009)

    Article  CAS  PubMed  Google Scholar 

  26. S. Dai, X. Wei, A.A. Alfonso, L. Pei, U.G. Duque, Z. Zhang, G.M. Babb, R.N. Beachy, Proc. Natl Acad. Sci. USA 105, 21012–21016 (2008)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. M. Ghildiyal, P.D. Zamore, Nat. Rev. Gen. 10, 94–108 (2009)

    Article  CAS  Google Scholar 

  28. L. Joshua-Tor, G.J. Hannon, Cold Spring Harb. Perspect. Biol. 3, a003772 (2011)

    Article  PubMed  PubMed Central  Google Scholar 

  29. R. Rajeswaran, M.M. Pooggin, MicroRNAs in Plant Development and Stress Response (Springer, Berlin, 2012), pp. 261–289

    Book  Google Scholar 

  30. A.J. Hamilton, D.C. Baulcombe, Science 286, 950–952 (1999)

    Article  CAS  PubMed  Google Scholar 

  31. S. Roy, A. Banerjee, J. Tarafdar, K.B. Senapati, I. Dasgupta, J. Agric. Sci. 150(5), 610–618 (2012)

    Article  CAS  Google Scholar 

  32. S. Roy, A. Banerjee, J. Tarafdar, K.B. Senapati, J. Genet. 91, 213–218 (2012)

    Article  PubMed  Google Scholar 

  33. M. Jyothsna, S. Manonmani, R. Rabindran, I. Dasgupta, S. Robin, Madras Agric J. 100(1–3), 70–74 (2013)

    Google Scholar 

  34. M. Jyothsna, S. Manonmani, M. Pradeep, Trends Biosci. 7(3), 149–152 (2014)

    Google Scholar 

  35. N. Nath, S. Mathur, I. Dasgupta, Arch. Virol. 147, 1173–1187 (2002)

    Article  CAS  PubMed  Google Scholar 

  36. V. Verma, I. Dasgupta, Arch. Virol. 152, 645–648 (2007)

    Article  CAS  PubMed  Google Scholar 

  37. H. Hibino, R.D. Daquioag, E.M. Mesina, V.M. Aguiero, Plant Dis. 74, 923–926 (1990)

    Article  Google Scholar 

  38. IRRI Standard Evaluation System (SES). Standard Evaluation System for Rice (INGER, IRRI, Philippines, 1996), 52p

    Google Scholar 

  39. S. Sharma, I. Dasgupta, J. Virol. Methods 181, 86–92 (2012)

    Article  CAS  PubMed  Google Scholar 

  40. M.A. Lodhi, G.N. Ye, N.F. Weeden, B.I. Reich, Plant Mol. Biol. Rep. 12, 6–13 (1994)

    Article  CAS  Google Scholar 

  41. T. Blevins, R. Rajeswaran, M. Aregger, B.K. Borah, M. Schepetilinikov, L. Baerlocher, L. Farinelli, F. Meins Jr, T. Hohn, M.M. Pooggin, Nucleic Acids Res. 39, 5003–5014 (2011)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  42. T. Csorba, L. Kontra, J. Burgyan, Virology 479–480, 85–103 (2015)

    Article  PubMed  Google Scholar 

  43. E. Di Nicola, M. Tavazza, A. Lucioli, L. Salindri, V. Ilardi, Mol. Plant Pathol. 15, 841–847 (2014)

    Article  PubMed  Google Scholar 

  44. J. Ye, J. Qu, H.Z. Mao, Z.G. Ma, N.E. Rahman, C. Bai, W. Chen, S.Y. Jiang, S. Ramachandran, N.H. Chua, Biotechnol. Biofuels 7(1), 149 (2015). doi:10.1186/s13068-014-0149-z

    Article  Google Scholar 

  45. L. Gao, X. Ding, K. Li, W. Liao, Y. Zhong, R. Ren, Z. Liu, K. Adhimoolam, H. Zhi, Theor. Appl. Genet. 128, 1489–1505 (2015)

    Article  CAS  PubMed  Google Scholar 

  46. J. Guo, S. Gao, Q. Lin, H. Wang, Y. Que, L. Xu et al., BioMed Res. Int. (2015). doi:10.1155/2015/86190

    Google Scholar 

  47. V.O. Ntui, K. Kong, R.S. Khan, T. Igawa, G.J. Janavi, R. Rabindran, I. Nakamura, M. Mii, PLoS One 10(4), e0120551 (2015)

    Article  PubMed  PubMed Central  Google Scholar 

  48. J. Fütterer, I. Potrykus, M.P. Valles Brau, I. Dasgupta, R. Hull, T. Hohn, Virology 198, 663–670 (1994)

    Article  PubMed  Google Scholar 

  49. A.R. Naqvi, M. Sarwat, B. Pradhan, N.R. Choudhury, Q.M. Haq, S.K. Mukherjee, Virus Res. 160, 395–399 (2011)

    Article  CAS  PubMed  Google Scholar 

  50. K. Satoh, H. Kondoh, T.B. De Leon, R.J.A. Macalalad, R.C. Cabunagan, P.Q. Cabauatan, R. Mauleon, S. Kikuchi, I.R. Choi, Virus Res. 171, 111–120 (2013)

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

DST INSPIRE Fellowship from Department of Science and Technology, Government of India and Research Fellowship from University Grants Commission, Government of India are gratefully acknowledged by VP and GK, respectively.

Funding

This work was funded by Department of Biotechnology, Government of India (Grant No. BT/PR-15033/AGR/02/773/2011).

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Authors and Affiliations

  1. Department of Plant Pathology, ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030, India

    P. Valarmathi

  2. Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, 641003, India

    R. Rabindran

  3. Department of Rice, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, 641003, India

    S. Robin & S. Manonmani

  4. Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, 110021, India

    G. Kumar & I. Dasgupta

Authors
  1. P. Valarmathi
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  2. G. Kumar
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  3. S. Robin
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  4. S. Manonmani
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  5. I. Dasgupta
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  6. R. Rabindran
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Correspondence to I. Dasgupta.

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Edited by Seung-Kook Choi.

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Valarmathi, P., Kumar, G., Robin, S. et al. Evaluation of virus resistance and agronomic performance of rice cultivar ASD 16 after transfer of transgene against Rice tungro bacilliform virus by backcross breeding. Virus Genes 52, 521–529 (2016). https://doi.org/10.1007/s11262-016-1318-x

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  • DOI: https://doi.org/10.1007/s11262-016-1318-x

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