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Effect of aged bone marrow microenvironment on mesenchymal stem cell migration

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Abstract

Mesenchymal stem cells (MSCs) are known to have many notable features, especially their multiple differentiation ability and immunoregulatory capacity. MSCs are important stem cells in the bone marrow (BM), and their characteristics are affected by the BM microenvironment. However, effects of the BM microenvironment on the properties of MSCs are not well understood. In this study, we found that BM from aged mice decreased MSC colony formation. Flow cytometry data showed that the proportion of B220+ cells in BM from aged mice was significantly lower than that in BM from young mice, while the proportion of CD11b+, CD3+, Gr-1+, or F4/80+ cells are on the contrary. CD11b+, B220+, and Ter119+ cells from aged mice were not the subsets that decreased MSC colony formation. We further demonstrated that both BM from aged mice and young mice exhibited similar effects on the proliferation of murine MSC cell line C3H10T1/2. However, when cocultured with BM from aged mice, C3H10T1/2 showed slower migration ability. In addition, we found that phosphorylation of JNK (c-Jun N-terminal kinases) in C3H10T1/2 cocultured with BM from aged mice was lower than that in C3H10T1/2 cocultured with BM from young mice. Collectively, our data revealed that BM from aged mice could decrease the migration of MSCs from their niche through regulating the JNK pathway.

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Acknowledgments

The authors wish to thank Lindsey Jones (Keck School of Medicine, University of Southern California) for her critical reading of the paper. This study was supported by grants from the National Natural Science Foundation of China (81271936), the Chinese National Key Program on Basic Research (2010CB529904).

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    Authors and Affiliations

    1. Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing, 100850, People’s Republic of China

      Yan-Mei Yang, Ping Li, Rui-Jie Dang & Ning Wen

    2. Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Science, 27 Taiping Road, Haidian District, Beijing, 100850, People’s Republic of China

      Yan-Mei Yang, Ping Li, Rui-Jie Dang, Lei Zhang & Xiao-Xia Jiang

    3. Beijing Aiyuhua Hospital for Children and Women, 2 South Street, Beijing Economic and Technological Development Zone, Beijing, 100176, People’s Republic of China

      Dian-Chao Cui

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    1. Yan-Mei Yang
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    4. Rui-Jie Dang
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    Correspondence to Ning Wen or Xiao-Xia Jiang.

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    Yan-Mei Yang and Ping Li contributed equally to this work.

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    Yang, YM., Li, P., Cui, DC. et al. Effect of aged bone marrow microenvironment on mesenchymal stem cell migration. AGE 37, 16 (2015). https://doi.org/10.1007/s11357-014-9743-z

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    • DOI: https://doi.org/10.1007/s11357-014-9743-z

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