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Short exposure to paclitaxel induces multipolar spindle formation and aneuploidy through promotion of acentrosomal pole assembly

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Abstract

Paclitaxel is a widely used microtubule drug and cancer medicine. Here we report that by short exposure to paclitaxel at a low dose, multipolar spindles were induced in mitotic cells without centrosome amplification. Both TPX2 depletion and Aurora-A overexpression antagonized the multipolarity. Live cell imaging showed that some paclitaxel-treated cells accomplished multipolar cell division and a portion of the daughter cells went on to the next round of mitosis. The surviving cells grew into clones with varied genome content. The results indicated that an aneuploidy population could be induced by short exposure to paclitaxel at a low dose, implicating potential side effects of paclitaxel.

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Authors and Affiliations

  1. MOE Key Laboratory of Cell Proliferation and Differentiation and the State Key Laboratory of Bio-membrane and Membrane Bio-engineering, College of Life Sciences, Peking University, Beijing, 100871, China

    MingLei Bian, JingYan Fu, Yan Yan, Qiang Chen, Qing Jiang & ChuanMao Zhang

  2. Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, 230026, China

    Chao Yang & QingHua Shi

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  1. MingLei Bian
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  2. JingYan Fu
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  3. Yan Yan
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  4. Qiang Chen
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  5. Chao Yang
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  7. Qing Jiang
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  8. ChuanMao Zhang
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Correspondence to ChuanMao Zhang.

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Bian, M., Fu, J., Yan, Y. et al. Short exposure to paclitaxel induces multipolar spindle formation and aneuploidy through promotion of acentrosomal pole assembly. Sci. China Life Sci. 53, 1322–1329 (2010). https://doi.org/10.1007/s11427-010-4086-1

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  • DOI: https://doi.org/10.1007/s11427-010-4086-1

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