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Basic Fibroblast Growth Factor Attenuates Bisphosphonate-Induced Oxidative Injury but Decreases Zinc and Copper Levels in Oral Epithelium of Rat

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Abstract

Recent studies have reported oxidative damage due to bisphosphonate (BP) in various cancer tissues and neurons, although basic fibroblast growth factor (bFGF) induced antioxidant effects in the cells. The bFGF may modulate the BP-induced oxidative stress in oral epithelium of rats. This study was undertaken to explore possible beneficial antioxidant effects of bFGF on oxidative stress induced by BP in oral epithelium of rats. Twenty-eight rats were equally divided into four groups. The first group was used as control. The second, third and fourth groups intraperitoneally received BP (zoledronic acid), bFGF and BP + bFGF. At the end of 10 weeks, the rats were sacrificed, and oral epithelium samples were taken for analyses. In BP group, the lipid peroxidation levels were increased in the oral epithelium, while the activities of glutathione peroxidase (GSH-Px) and the concentrations of total antioxidant status (TAS) were decreased. In rats treated with bFGF, lipid peroxidation levels decreased, and the activities of GSH-Px and concentrations of TAS improved in the oral epithelium. However, zinc and copper levels were decreased in the oral epithelium by BP and bFGF treatments. Concentrations of vitamin E and reduced glutathione in the samples did not change in the groups. In conclusion, treatment with bFGF modulated the antioxidant redox system and reduced the oral epithelium oxidative stress induced by BP. However, zinc and copper levels were decreased by BP and bFGF treatments.

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Abbreviations

bFGF:

Basic fibroblast growth factor

BP:

Bisphosphonate

Cu:

Copper

GSH:

Glutathione

GSH-Px:

Glutathione peroxidase

MDA:

Malondialdehyde

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

TAS:

Total antioxidant status

Zn:

Zinc

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Conflict of Interest

There is no conflict interest and financial support in the current study.

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, Dentistry Faculty, Suleyman Demirel University, Isparta, Turkey

    Gülperi Koçer & Levent Önal

  2. Neuroscience Research Center, Suleyman Demirel University, Tıp Fakültesi Binasi, TR-32260, Isparta, Turkey

    Mustafa Nazıroğlu & Ömer Çelik

  3. Department of Biophysics, Medical Faculty, Suleyman Demirel University, Isparta, Turkey

    Mustafa Nazıroğlu & Ömer Çelik

  4. Department of Biophysics, Medical Faculty, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey

    Derviş Özçelik

  5. Department of Medical Oncology, Medical Faculty, Suleyman Demirel University, Isparta, Turkey

    Murat Koçer

  6. Department of Oral and Maxillofacial Surgery, Medical Faculty, RWTH Aachen University, Aachen, Germany

    Tolga Taha Sönmez

Authors
  1. Gülperi Koçer
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  2. Mustafa Nazıroğlu
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  3. Ömer Çelik
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  4. Levent Önal
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  5. Derviş Özçelik
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  6. Murat Koçer
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  7. Tolga Taha Sönmez
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Correspondence to Mustafa Nazıroğlu.

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Koçer, G., Nazıroğlu, M., Çelik, Ö. et al. Basic Fibroblast Growth Factor Attenuates Bisphosphonate-Induced Oxidative Injury but Decreases Zinc and Copper Levels in Oral Epithelium of Rat. Biol Trace Elem Res 153, 251–256 (2013). https://doi.org/10.1007/s12011-013-9659-y

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  • DOI: https://doi.org/10.1007/s12011-013-9659-y

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