Skip to main content

Advertisement

SpringerLink
Log in

Nephroprotective Role of Selenium Nanoparticles Against Glycerol-Induced Acute Kidney Injury in Rats

  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

Abstract

Acute kidney injury (AKI) is a clinical syndrome associated with the incidence of rhabdomyolysis (RM). The current study was carried out to evaluate whether selenium nanoparticles (SeNPs) can protect against the glycerol-induced AKI model. Rats were distributed into four equal groups (n = 7): the control group (G1), SeNPs group (G2), AKI group (G3), and SeNPs+AKI group (G4). Rats in G1 were intramuscularly injected with physiological saline (0.9% NaCl). Rats in G2 were gavaged with SeNPs (0.1 mg/kg) for 14 days. Rats in G3 were intramuscularly injected with 50% glycerol (10 ml/kg). Rats in G4 were administered with SeNPs for 14 days and then injected with glycerol, as in G3. Glycerol-injected rats showed a significant increase in the kidney relative weight, as well as in the serum urea, creatinine, Kim-1, and renal malondialdehyde, nitric oxide, TNF-α, IL-1β, cytochrome c, Bax, and caspase-3 levels. In addition, a significant decrease in glutathione, glutathione peroxidase, glutathione reductase, superoxide dismutase, and catalase was recorded in the renal tissue. Selenium nanoparticles reduced the biochemical, molecular, and histological changes produced by glycerol. Overall, our results suggest that selenium nanoparticles could be used to protect against AKI development via antioxidant, anti-inflammatory, and anti-apoptotic activities.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Shi Y, Xu L, Tang J, Fang L, Ma S, Ma X, Nie J, Pi X, Qiu A, Zhuang S, Liu N (2017) Inhibition of HDAC6 protects against rhabdomyolysis-induced acute kidney injury. Am J Physiol Ren Physiol 312(3):F502–F515. https://doi.org/10.1152/ajprenal.00546.2016

    Article  CAS  Google Scholar 

  2. Bosch X, Poch E, Grau JM (2009) Rhabdomyolysis and acute kidney injury. N Engl J Med 361(1):62–72. https://doi.org/10.1056/NEJMra0801327

    Article  CAS  PubMed  Google Scholar 

  3. Petejova N, Martinek A (2014) Acute kidney injury due to rhabdomyolysis and renal replacement therapy: a critical review. Crit Care 18(3):224. https://doi.org/10.1186/cc13897

    Article  PubMed  PubMed Central  Google Scholar 

  4. Korrapati MC, Shaner BE, Schnellmann RG (2012) Recovery from glycerol-induced acute kidney injury is accelerated by suramin. J Pharmacol Exp Ther 341(1):126–136. https://doi.org/10.1124/jpet.111.190249

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Singh AP, Junemann A, Muthuraman A, Jaggi AS, Singh N, Grover K, Dhawan R (2012) Animal models of acute renal failure. Pharmacol Rep 64(1):31–44

    Article  CAS  PubMed  Google Scholar 

  6. Al Asmari AK, Al Sadoon KT, Obaid AA, Yesunayagam D, Tariq M (2017) Protective effect of quinacrine against glycerol-induced acute kidney injury in rats. BMC Nephrol 18(1):41. https://doi.org/10.1186/s12882-017-0450-8

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Boutaud O, Moore KP, Reeder BJ, Harry D, Howie AJ, Wang S, Carney CK, Masterson TS, Amin T, Wright DW, Wilson MT, Oates JA, Roberts LJ 2nd (2010) Acetaminophen inhibits hemoprotein-catalyzed lipid peroxidation and attenuates rhabdomyolysis-induced renal failure. Proc Natl Acad Sci U S A 107(6):2699–2704. https://doi.org/10.1073/pnas.0910174107

    Article  PubMed  PubMed Central  Google Scholar 

  8. Iglesias P, Selgas R, Romero S, Diez JJ (2013) Selenium and kidney disease. J Nephrol 26(2):266–272. https://doi.org/10.5301/jn.5000213

    Article  CAS  PubMed  Google Scholar 

  9. Bai K, Hong B, Hong Z, Sun J, Wang C (2017) Selenium nanoparticles-loaded chitosan/citrate complex and its protection against oxidative stress in d-galactose-induced aging mice. J Nanobiotechnol 15(1):92. https://doi.org/10.1186/s12951-017-0324-z

    Article  CAS  Google Scholar 

  10. Krishnan V, Loganathan C, Thayumanavan P (2019) Green synthesized selenium nanoparticles using Spermacoce hispida as carrier of s-allyl glutathione: to accomplish hepatoprotective and nephroprotective activity against acetaminophen toxicity. Artif Cells Nanomed Biotechnol 47(1):56–63. https://doi.org/10.1080/21691401.2018.1543192

    Article  CAS  PubMed  Google Scholar 

  11. Loeschner K, Hadrup N, Hansen M, Pereira SA, Gammelgaard B, Moller LH, Mortensen A, Lam HR, Larsen EH (2014) Absorption, distribution, metabolism and excretion of selenium following oral administration of elemental selenium nanoparticles or selenite in rats. Metallomics 6(2):330–337. https://doi.org/10.1039/c3mt00309d

    Article  CAS  PubMed  Google Scholar 

  12. Al-Quraishy S, Dkhil MA, Abdel Moneim AE (2015) Anti-hyperglycemic activity of selenium nanoparticles in streptozotocin-induced diabetic rats. Int J Nanomedicine 10:6741–6756. https://doi.org/10.2147/IJN.S91377

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Kim JH, Lee SS, Jung MH, Yeo HD, Kim HJ, Yang JI, Roh GS, Chang SH, Park DJ (2010) N-acetylcysteine attenuates glycerol-induced acute kidney injury by regulating MAPKs and Bcl-2 family proteins. Nephrol Dial Transplant 25(5):1435–1443

    Article  CAS  PubMed  Google Scholar 

  14. Almeer RS, AlBasher GI, Alarifi S, Alkahtani S, Ali D, Abdel Moneim AE (2019) Royal jelly attenuates cadmium-induced nephrotoxicity in male mice. Sci Rep 9(1):5825. https://doi.org/10.1038/s41598-019-42368-7

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193(1):265–275

    CAS  PubMed  Google Scholar 

  16. Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95(2):351–358

    Article  CAS  PubMed  Google Scholar 

  17. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR (1982) Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal Biochem 126(1):131–138

    Article  CAS  PubMed  Google Scholar 

  18. Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82(1):70–77

    Article  CAS  PubMed  Google Scholar 

  19. Nishikimi M, Appaji N, Yagi K (1972) The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res Commun 46(2):849–854

    Article  CAS  PubMed  Google Scholar 

  20. Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126

    Article  CAS  PubMed  Google Scholar 

  21. Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70(1):158–169

    CAS  PubMed  Google Scholar 

  22. De Vega L, Fernandez RP, Mateo MC, Bustamante JB, Herrero AM, Munguira EB (2002) Glutathione determination and a study of the activity of glutathione-peroxidase, glutathione-transferase, and glutathione-reductase in renal transplants. Ren Fail 24(4):421–432

    Article  PubMed  Google Scholar 

  23. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25(4):402–408. https://doi.org/10.1006/meth.2001.1262

    Article  CAS  PubMed  Google Scholar 

  24. Abdel Moneim AE (2016) Indigofera oblongifolia prevents lead acetate-induced hepatotoxicity, oxidative stress, fibrosis and apoptosis in rats. PLoS One 11(7):e0158965. https://doi.org/10.1371/journal.pone.0158965

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Mousleh R, Al Laham S, Al-Manadili A (2018) The preventive role of pioglitazone in glycerol-induced acute kidney injury in rats during two different treatment periods. Iran J Med Sci 43(2):184–194

    PubMed  PubMed Central  Google Scholar 

  26. Shanu A, Groebler L, Kim HB, Wood S, Weekley CM, Aitken JB, Harris HH, Witting PK (2013) Selenium inhibits renal oxidation and inflammation but not acute kidney injury in an animal model of rhabdomyolysis. Antioxid Redox Signal 18(7):756–769. https://doi.org/10.1089/ars.2012.4591

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Al-Brakati AY, Kassab RB, Lokman MS, Elmahallawy EK, Amin HK, Abdel Moneim AE (2018) Role of thymoquinone and ebselen in the prevention of sodium arsenite-induced nephrotoxicity in female rats. Hum Exp Toxicol 38:482–493. https://doi.org/10.1177/0960327118818246

    Article  CAS  PubMed  Google Scholar 

  28. Sharawy MH, Abdelrahman RS, El-Kashef DH (2018) Agmatine attenuates rhabdomyolysis-induced acute kidney injury in rats in a dose dependent manner. Life Sci 208:79–86. https://doi.org/10.1016/j.lfs.2018.07.019

    Article  CAS  PubMed  Google Scholar 

  29. Yalcinkaya Yavuz O, Aydogdu N, Tastekin E, Sut N (2018) The Effects of baicalin on myoglobinuric acute renal failure in rats. Balkan Med J 35(1):68–76. https://doi.org/10.4274/balkanmedj.2017.0040

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Kassab RB, Lokman MS, Essawy EA (2018) Neurochemical alterations following the exposure to di-n-butyl phthalate in rats. Metab Brain Dis 34:235–244. https://doi.org/10.1007/s11011-018-0341-0

    Article  CAS  PubMed  Google Scholar 

  31. Al-Olayan EM, El-Khadragy MF, Omer SA, Shata MT, Kassab RB, Abdel Moneim AE (2016) The beneficial effect of cape gooseberry juice on carbon tetrachloride-induced neuronal damage. CNS Neurol Disord Drug Targets 15(3):344–350

    Article  CAS  PubMed  Google Scholar 

  32. Sun X, Luan Q, Qiu S (2018) Valsartan prevents glycerol-induced acute kidney injury in male albino rats by downregulating TLR4 and NF-kappaB expression. Int J Biol Macromol 119:565–571. https://doi.org/10.1016/j.ijbiomac.2018.07.149

    Article  CAS  PubMed  Google Scholar 

  33. Shang Y, Siow YL, Isaak CK, O K (2016) Downregulation of glutathione biosynthesis contributes to oxidative stress and liver dysfunction in acute kidney injury. Oxidative Med Cell Longev 2016:9707292. https://doi.org/10.1155/2016/9707292

    Article  CAS  Google Scholar 

  34. Zhao B, Sun X, Gao X (2014) Pretreatment with hydrogen-rich saline reduces the damage caused by glycerol-induced rhabdomyolysis and acute kidney injury in rats. J Surg Res 188(1):243–249. https://doi.org/10.1016/j.jss.2013.12.007

    Article  CAS  PubMed  Google Scholar 

  35. Nishida K, Watanabe H, Ogaki S, Kodama A, Tanaka R, Imafuku T, Ishima Y, Chuang VT, Toyoda M, Kondoh M, Wu Q, Fukagawa M, Otagiri M, Maruyama T (2015) Renoprotective effect of long acting thioredoxin by modulating oxidative stress and macrophage migration inhibitory factor against rhabdomyolysis-associated acute kidney injury. Sci Rep 5:14471. https://doi.org/10.1038/srep14471

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Bizerea TO, Dezsi SG, Marginean O, Stroescu R, Rogobete A, Bizerea-Spiridon O, Ilie C (2018) The link between selenium, oxidative stress and pregnancy induced hypertensive disorders. Clin Lab 64(10):1593–1610. https://doi.org/10.7754/Clin.Lab.2018.180307

    Article  CAS  PubMed  Google Scholar 

  37. Dkhil MA, Zrieq R, Al-Quraishy S, Abdel Moneim AE (2016) Selenium nanoparticles attenuate oxidative stress and testicular damage in streptozotocin-induced diabetic rats. Molecules 21(11). https://doi.org/10.3390/molecules21111517

    Article  PubMed Central  Google Scholar 

  38. Rayman MP (2000) The importance of selenium to human health. Lancet 356(9225):233–241. https://doi.org/10.1016/S0140-6736(00)02490-9

    Article  CAS  PubMed  Google Scholar 

  39. Kunak CS, Ugan RA, Cadirci E, Karakus E, Polat B, Un H, Halici Z, Saritemur M, Atmaca HT, Karaman A (2016) Nephroprotective potential of carnitine against glycerol and contrast-induced kidney injury in rats through modulation of oxidative stress, proinflammatory cytokines, and apoptosis. Br J Radiol 89(1058):20140724. https://doi.org/10.1259/bjr.20140724

    Article  PubMed  Google Scholar 

  40. Homsi E, Janino P, de Faria JB (2006) Role of caspases on cell death, inflammation, and cell cycle in glycerol-induced acute renal failure. Kidney Int 69(8):1385–1392. https://doi.org/10.1038/sj.ki.5000315

    Article  CAS  PubMed  Google Scholar 

  41. Gois PHF, Canale D, Volpini RA, Ferreira D, Veras MM, Andrade-Oliveira V, Camara NOS, Shimizu MHM, Seguro AC (2016) Allopurinol attenuates rhabdomyolysis-associated acute kidney injury: renal and muscular protection. Free Radic Biol Med 101:176–189. https://doi.org/10.1016/j.freeradbiomed.2016.10.012

    Article  CAS  PubMed  Google Scholar 

  42. Khurana A, Tekula S, Saifi MA, Venkatesh P, Godugu C (2019) Therapeutic applications of selenium nanoparticles. Biomed Pharmacother 111:802–812. https://doi.org/10.1016/j.biopha.2018.12.146

    Article  CAS  PubMed  Google Scholar 

  43. Havasi A, Borkan SC (2011) Apoptosis and acute kidney injury. Kidney Int 80(1):29–40. https://doi.org/10.1038/ki.2011.120

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  44. Brooks C, Wei Q, Cho SG, Dong Z (2009) Regulation of mitochondrial dynamics in acute kidney injury in cell culture and rodent models. J Clin Invest 119(5):1275–1285. https://doi.org/10.1172/JCI37829

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

The authors extend their appreciation to the Deanship of Scientific Research and RSSU-RSSUF at King Saud University for the technical support and language editing.

Funding

This study was financially supported by the Deanship of Scientific Research at King Saud University (research group no. RG-1439-60).

Author information

Authors and Affiliations

  1. Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia

    Gadah AlBasher, Saleh Alfarraj, Saud Alarifi, Saad Alkhtani, Rafa Almeer & Nouf Alsultan

  2. Department of Applied Medical Science, Collage of Applied Medical Science, Shaqra University, Riyadh, Saudi Arabia

    Mada Alharthi

  3. Department of Chemistry, College of Sciences, King Saud University, Riyadh, Saudi Arabia

    Nouf Alotibi

  4. Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia

    Abeer Al-dbass

  5. Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt

    Ahmed E. Abdel Moneim

Authors
  1. Gadah AlBasher
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Saleh Alfarraj
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Saud Alarifi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Saad Alkhtani
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rafa Almeer
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Nouf Alsultan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mada Alharthi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Nouf Alotibi
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Abeer Al-dbass
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Ahmed E. Abdel Moneim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gadah AlBasher.

Ethics declarations

Conflict of Interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Figure S1

Morphological characterization of SeNPs freshly synthesized in aqueous solution. A: EDX analysis of one SeNP of panel. B-C: Measurement of size distribution of SeNPs by dynamic light scattering. (JPG 214 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

AlBasher, G., Alfarraj, S., Alarifi, S. et al. Nephroprotective Role of Selenium Nanoparticles Against Glycerol-Induced Acute Kidney Injury in Rats. Biol Trace Elem Res 194, 444–454 (2020). https://doi.org/10.1007/s12011-019-01793-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12011-019-01793-5

Keywords

Search

Navigation