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Establishment of a Rat Model of Adjuvant-Induced Osteoarthritis of the Lumbar Facet Joint

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Abstract

To study the establishment of adjuvant-induced osteoarthritis of the lumbar facet joint in a rat model. Complete Freund’s adjuvant (experimental group) and saline (control group) were randomly injected into the right and left side of rat, respectively. The rats were killed, and degeneration of lumbar facet joint was evaluated at macroscopic level and scored based on OARSI scores system. Moreover, Interleukin-1β and tumor necrosis factor-α levels in the synovium were measured. The macroscopic scores and OARSI scores of experimental group were higher than the control group (P < 0.05). The concentration of tumor necrosis factor-α was significantly increased only on 3- and 7-day post-surgery when compared with controls, and interleukin-1β was increased on days 3,7 and 14 post-surgery (P < 0.05). The rat model of adjuvant can induce degeneration of the lumbar facet joint. It can be useful for studies on mechanisms and treatment of lumbar facet joint osteoarthritis.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 81071514).

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Authors and Affiliations

  1. Department of Orthopaedics, The First Affiliated Hospital of Chinese PLA General Hospital, Beijing, 100048, China

    Feng Shuang, Jialiang Zhu, Keran Song, Shuxun Hou, Yan Liu, Chunli Zhang & Jiaguang Tang

  2. Department of Orthopaedics, The 94th Hospital of Chinese PLA, Nanchang, 330002, China

    Feng Shuang

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  1. Feng Shuang
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  2. Jialiang Zhu
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  3. Keran Song
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  4. Shuxun Hou
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  5. Yan Liu
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  6. Chunli Zhang
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  7. Jiaguang Tang
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Correspondence to Shuxun Hou or Jiaguang Tang.

Additional information

Feng Shuang, Jialiang Zhu and Keran Song have contributed equally to this study.

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Shuang, F., Zhu, J., Song, K. et al. Establishment of a Rat Model of Adjuvant-Induced Osteoarthritis of the Lumbar Facet Joint. Cell Biochem Biophys 70, 1545–1551 (2014). https://doi.org/10.1007/s12013-014-0091-5

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