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Human Periosteal Derived Stem Cell Potential: The Impact of age

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Abstract

There is a great deal of interest in the understanding of possible age-related changes in Mesenchymal Stem Cells in view of their use for regenerative medicine applications. Given to the outmost standing of periosteum in bone biology and to probe data for a cell-based therapy promoting graft osseointegration in the elderly, we tried to identify specific aging markers or pattern of expression in human periosteal precursor cells. Immunohistochemical detection of Ki67 and p53, Nitric Oxide production and qRT- PCR of a selected gene panel for osteoblastic differentiation, bone remodeling and stemness were evaluated. We confirmed that both Ki67 and p53 are noteworthy indicators of senescence in human periosteal precursor cells and their expression significantly correlate with cell NO production. Moreover, cell age affects genes involved in bone remodeling, with a significant increase in interleukin-6 mRNA expression and receptor activator of nuclear factor kappa-B ligand/osteoprotegerin ratio. The analysis of mRNAs of genes involved in pluripotency regulation and self-renewal of stem cells, evidenced changes at least in part related to bone remodeling. We believe that this is the first study taking on age-related changes in human periosteal precursor cells, and paving the way toward new regenerative medicine strategies in bone aging and/or bone metabolic diseases.

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Acknowledgments

This contribution is cofounded by the Italian Ministry of Education, University & Research (MIUR) (Project PRIN 2010, MIND, 2010J8RYS7).

Each author certifies that he or she has no commercial associations (e.g., consultancies, stock ownership, equity interest, patent/licensing arrangements, etc.) that might pose a conflict of interest in connection with the submitted article

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Authors and Affiliations

  1. Department of Clinical and Molecular Sciences, Università Politecnica delle Marche, Via Tonto 10/a, 60126, Ancona, Italy

    Concetta Ferretti, Guendalina Lucarini, Eleonora Salvolini, Novella Bianchi, Antonio Gigante & Monica Mattioli-Belmonte

  2. Research Centre “E. Piaggio” Faculty of Engineering, University of Pisa, Pisa, Italy

    Chiara Andreoni & Giovanni Vozzi

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  1. Concetta Ferretti
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  2. Guendalina Lucarini
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  3. Chiara Andreoni
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  4. Eleonora Salvolini
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  5. Novella Bianchi
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  6. Giovanni Vozzi
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  7. Antonio Gigante
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  8. Monica Mattioli-Belmonte
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Correspondence to Monica Mattioli-Belmonte.

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Ferretti, C., Lucarini, G., Andreoni, C. et al. Human Periosteal Derived Stem Cell Potential: The Impact of age. Stem Cell Rev and Rep 11, 487–500 (2015). https://doi.org/10.1007/s12015-014-9559-3

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