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Antimicrobial activity and biofilm formation inhibition of green tea polyphenols on human teeth

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Abstract

The antimicrobial effects and biofilm formation inhibition of tea polyphenols (TPP) extracted from Korean green tea (Camellia sinensis L) were evaluated against 12 oral microorganisms. Effective antimicrobial activity against all microorganisms tested, including Lactobacillus spp. (Lactobacillus acidophilus and Lactobacillus plantarum), Streptococcus spp. (Streptococcus mutans, Streptococcus sanguis, Streptococcus sobrinus, Streptococcus mitis, and Streptococcus salivarius), Staphylococcus aureus, Neisseria meningitidis, Escherichia coli, Enterobacter cloacae, Enterococcus faecalis, and Candida albicans, was shown at 2,000 μg/mL TPP within 5 min of incubation. Scanning electron microscopy (SEM) analysis revealed various morphological changes, such as the presence of perforations, the formation of cell aggregates, and the leakage of cytoplasmic materials from cells treated with TPP, depending on the bacteria. The potential role of TPP in biofilm formation inhibition on human teeth was evaluated in BHI broth with 2 mixed strains of S. mutans and S. sanguis. SEM analysis showed biofilm formation on the surface of a tooth shaken only in saline solution, whereas almost no biofilm was observed on a tooth incubated in TPP solution. This result suggests that TPP is effective against adherent cells of S. mutans and S. sanguis. Thus, TPP would be useful for development as an antimicrobial agent against oral microorganisms, and has great potential for use in mouthwash solutions for the prevention and treatment of dental caries.

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Authors and Affiliations

  1. Department of Biotechnology, Soonchunhyang University, Chung-Nam, 336-600, Korea

    Yun-Seok Cho & Kye-Heon Oh

  2. Program of Microbiology, Indiana University, Bloomington, IN, 47402, USA

    Jay Jooyoung Oh

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  1. Yun-Seok Cho
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  2. Jay Jooyoung Oh
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Correspondence to Kye-Heon Oh.

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Cho, YS., Oh, J.J. & Oh, KH. Antimicrobial activity and biofilm formation inhibition of green tea polyphenols on human teeth. Biotechnol Bioproc E 15, 359–364 (2010). https://doi.org/10.1007/s12257-009-0195-8

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  • DOI: https://doi.org/10.1007/s12257-009-0195-8

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