Skip to main content
Log in

Facile 3D cell culture protocol based on photocurable hydrogels

  • Technical Note
  • Published:
Bio-Design and Manufacturing Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

References

  1. Zhu Z, Liu J-D, Liu C, Wu X, Li Q, Chen S, Zhao X, Weitz DA (2020) Microfluidics-assisted assembly of injectable photonic hydrogels toward reflective cooling. Small 16(9):1903939

    Article  Google Scholar 

  2. Jensen C, Teng Y (2020) Is it time to start transitioning from 2D to 3D cell culture? Front Mol Biosci 7:33

    Article  Google Scholar 

  3. De Leon SE, Pupovac A, McArthur SL (2020) Three-dimensional (3D) cell culture monitoring: opportunities and challenges for impedance spectroscopy. Biotechnol Bioeng 117(4):1230–1240

    Article  Google Scholar 

  4. Zhao X, Liu S, Yildirimer L, Zhao H, Ding R, Wang H, Cui W, Weitz D (2016) Injectable stem cell-laden photocrosslinkable microspheres fabricated using microfluidics for rapid generation of osteogenic tissue constructs. Adv FuncT Mater 26(17):2809–2819

    Article  Google Scholar 

  5. Xie M, Gao Q, Zhao H, Nie J, Fu Z, Wang H, Chen L, Shao L, Fu J, Chen Z, He Y (2019) Electro-assisted bioprinting of low-concentration GelMA microdroplets. Small 15(4):1804216

    Article  Google Scholar 

  6. Xie M, Gao Q, Qiu J, Fu J, Chen Z, He Y (2020) 3D biofabrication of microfiber-laden minispheroids: a facile 3D cell co-culturing system. Biomater Sci 8(1):109–117

    Article  Google Scholar 

  7. Xie M, Gao Q, Fu J, Chen Z, He Y (2020) Bioprinting of novel 3D tumor array chip for drug screening. Bio-Des Manuf 3(3):175–188

    Article  Google Scholar 

  8. Shao L, Gao Q, Xie C, Fu J, Xiang M, Liu Z, Xiang L, He Y (2020) Sacrificial microgel-laden bioink-enabled 3D bioprinting of mesoscale pore networks. Bio-Des Manuf 3(1):30–39

    Article  Google Scholar 

  9. Nie J, Gao Q, Wang Y, Zeng J, Zhao H, Sun Y, Shen J, Ramezani H, Fu Z, Liu Z, Xiang M, Fu J, Zhao P, Chen W, He Y (2018) Vessel-on-a-chip with hydrogel-based microfluidics. Small 14(45):1802368

    Article  Google Scholar 

  10. Xie M, Yu K, Sun Y, Shao L, Nie J, Gao Q, Qiu J, Fu J, Chen Z, He Y (2019) Protocols of 3D bioprinting of gelatin methacryloyl hydrogel based bioinks. JoVE 154:e60545

    Google Scholar 

  11. Yue K, Trujillo-de Santiago G, Alvarez MM, Tamayol A, Annabi N, Khademhosseini A (2015) Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels. Biomaterials 73:254–271

    Article  Google Scholar 

  12. Yoon HJ, Shin SR, Cha JM, Lee S-H, Kim J-H, Do JT, Song H, Bae H (2016) Cold water fish gelatin methacryloyl hydrogel for tissue engineering application. PLoS ONE 11(10):e0163902

    Article  Google Scholar 

  13. Yuan S, Kang Y, Jing N, Miao S, Jianzhong F, Huiming W, Yong H (2020) Modeling the printability of photocuring and strength adjustable hydrogel bioink during projection based 3D bioprinting. Biofabrication. https://doi.org/10.1088/1758-5090/aba413

    Article  Google Scholar 

  14. Zhao X, Lang Q, Yildirimer L, Lin ZY, Cui W, Annabi N, Ng KW, Dokmeci MR, Ghaemmaghami AM, Khademhosseini A (2016) Photocrosslinkable gelatin hydrogel for epidermal tissue engineering. Adv Healthc Mater 5(1):108–118

    Article  Google Scholar 

  15. Sun X, Lang Q, Zhang H, Cheng L, Zhang Y, Pan G, Zhao X, Yang H, Zhang Y, Santos HA, Cui W (2017) Electrospun photocrosslinkable hydrogel fibrous scaffolds for rapid in vivo vascularized skin flap regeneration. Adv Funct Mater 27(2):1604617

    Article  Google Scholar 

  16. Chen H, Guo L, Wicks J, Ling C, Zhao X, Yan Y, Qi J, Cui W, Deng L (2016) Quickly promoting angiogenesis by using a DFO-loaded photo-crosslinked gelatin hydrogel for diabetic skin regeneration. J Mater Chem B 4(21):3770–3781

    Article  Google Scholar 

  17. Prestwich GD (2007) Simplifying the extracellular matrix for 3-D cell culture and tissue engineering: a pragmatic approach. J Cell Biochem 101(6):1370–1383

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Nature Science Foundation of China (Nos. U1909218, 81827804).

Author information

Authors and Affiliations

Authors

Contributions

YH conceived the study; MX and YZ contributed to methodology and investigation; MX helped in writing original draft; all the authors contributed to writing and editing; YH contributed to funding acquisition; QG provided resources; YH supervised the study

Corresponding author

Correspondence to Yong He.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This study does not contain any work related to human or animal subjects performed by any of the authors.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 7338 kb)

Supplementary material 2 (MP4 28273 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Xie, M., Zheng, Y., Gao, Q. et al. Facile 3D cell culture protocol based on photocurable hydrogels. Bio-des. Manuf. 4, 149–153 (2021). https://doi.org/10.1007/s42242-020-00096-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42242-020-00096-2

Navigation