Abstract
Quercetin, a plant-derived aglycone form of flavonoid glycosides, has been used as a nutritional supplement and may be beneficial against a variety of diseases, including cancer. We examined the antioxidant properties of quercetin. The reduction potential of quercetin was measured at various pH values using voltammetric methods, and its total antioxidant capacity (TAC) was measured using the phosphomolybdenum method. The effect of quercetin on production of reactive oxygen species (ROS) and nitric oxide (NO) in LPS-stimulated human THP-1 acute monocytic leukemia cells was determined by flow cytometry using CM-H2DCFDA dye. The results were compared with curcumin, a natural product exhibiting a similar range of reported health benefits. Results: 1) Quercetin has a higher reduction potential compared with curcumin at three different pH settings and is comparable to Trolox at pH 7-9.5; 2) its TAC is 3.5 fold higher than curcumin; 3) it reduced LPS-induced ROS to near normal levels; 4) it reduced LPS-induced NO production. These data provide a physico-chemical basis for comparing antioxidants, with potential benefits individually or in combination.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Neuhouser ML (2004) Dietary flavonoids and cancer risk: evidence from human population studies. Nutr Cancer 50(1):1-7
Murakami A, Ashida H, Terao J (2008) Multitargeted cancer prevention by quercetin. Cancer Lett 269(2): 315-325
Shankar S, Singh G, Srivastava RK (2007) Chemoprevention by resveratrol: molecular mechanisms and therapeutic potential. Front Biosci 12:4839-4854
Labinskyy N, Csiszar A, Veress G, et al (2006) Vascular dysfunction in aging: potential effects of resveratrol, an anti-inflammatory phytoestrogen. Curr Med Chem 13:989-996.
Yang JY, Della-Fera MA, Rayalam S, et al (2008) Enhanced inhibition of adipogenesis and induction of apoptosis in 3T3-L1 adipocytes with combinations of resveratrol and quercetin. Life Sci 82(19-20):1032-1039
Nam NH (2006) Naturally occurring NF-kappaB inhibitors. Med Chem 6:945-951
Teixeira S (2002) Bioflavonoids: proanthocyanidins and quercetin and their potential roles in treating musculoskeletal conditions. J Orthop Sports Phys Ther 32(7):357-363.
GarcÃa-Mediavilla V, Crespo I, Collado PS, et al (2007) The anti-inflammatory flavones quercetin and kaempferol cause inhibition of inducible nitric oxide synthase, cyclooxygenase-2 and reactive C-protein, and down-regulation of the nuclear factor kappaB pathway in Chang Liver cells. Eur J Pharmacol 557(2-3):221-229
Karbarz M, Malyszko J (2008) Voltammetric behavior of trolox in methanol and ethanol solutions. Electroanalysis 20(17):1884-1890
Malyszko J, Karbarz M (2006) Electrochemical oxidation of trolox and a-tocopherol in acetic acid: A comparative study. J Electroanal Chem 595:136-144
Timbola AK, de Souza CD, Giacomelli C, et al (2006) Electrochemical oxidation of quercetin in hydroalcoholic solution. J Braz Chem Soc 17:139-148
Jovanovic SV, Steenken S, BooneCW, et al (1999) H-Atom Transfer IsAPreferred Antioxidant Mechanism of Curcumin. J Am Chem Soc 121:9677-9681
Mullen W, Rouanet JM, Auger C, et al (2008) Bioavailability of [2-(14)C]quercetin-4’- glucoside in rats. J Agric Food Chem 2456(24):12127-12137.
Verschoyle RD, Steward WP, Gescher AJ (2007) Putative cancer chemopreventive agents of dietary origin-how safe are they? Nutr Cancer 59(2):152-162.
van der Woude H, Alink GM, van Rossum BE, et al (2005) Formation of transient covalent protein and DNA adducts by quercetin in cells with and without oxidative enzyme activity. Chem Res Toxicol 18(12):1907-1916
Sun W,WangW,Kim J, et al (2008) Anti-cancer effect of resveratrol is associatedwith induction of apoptosis via a mitochondrial pathway alignment. Adv Exp Med Biol 614:179-186
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this paper
Cite this paper
Zhang, M. et al. (2011). Antioxidant Properties of Quercetin. In: LaManna, J., Puchowicz, M., Xu, K., Harrison, D., Bruley, D. (eds) Oxygen Transport to Tissue XXXII. Advances in Experimental Medicine and Biology, vol 701. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-7756-4_38
Download citation
DOI: https://doi.org/10.1007/978-1-4419-7756-4_38
Published:
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4419-7755-7
Online ISBN: 978-1-4419-7756-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)