Abstract
The human astroviruses (HAst) are increasingly recognized as an important cause of gastroenteritis. These viruses contain a 6.8-kb positive-sense, single-stranded RNA molecule that is infectious when transfected into permissive cells. The HAst gene 1 is composed of two open reading frames (ORFs 1a and 1b) connected by a ribosomal frameshift. Gene 1 is predicted to encode two nonstructural polyproteins (pp1a and pp1ab), and analysis of the HAs gene 1 sequence has resulted in predictions of a serine proteinase within the ORF1a polyprotein. However, none of the gene 1 proteins have been identified. To examine the expression and processing of the HAst2 gene 1 polyprotein, we have translated pp1a and pp1ab in vitro. These ongoing studies will provide the foundation for correlating gene 1 expression in vitro with proteins expressed in virus-infected cells.
Chapter PDF
Similar content being viewed by others
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Carter, M.J., 1994, Genomic organization and expression of astroviruses and caliciviruses, Arch. Virol. Supp. 9: 429–439.
Geigenmuller, U., Ginzton, N., and Matsui, S., 1996, Construction of a genome-length cDNA clone for human astrovirus serotype 1 and synthesis of infectious RNA transcripts, J. Virol. 71: 1713–1717.
Jiang, B., Monroe, S.S., Koonin, E.V., Stine, S.E., and Glass, R.I., 1993, RNA sequence of astrovirus: distinctive genomic organization and a putative retrovirus-like ribosomal frameshifting signal that directs the viral replicase synthesis, Proc. Nat. Acad. Sci. 90: 10539–10543.
Lewis, T., and Matsui, S., 1996, Astrovirus ribosomal frameshifting in an infection-transfection transient expression system, J. Virol. 70: 2869–2875.
Madeley, C.R., Cosgrove, B.P., and Bell, E.J., Stool viruses in babies in Glasgow2: Investigation of normal newborns in hospital, J. Hygiene 81: 285–294.
Matsui, S.M., Kim, J.P., Greenberg, H.B., Young, L.M., Smith, L.S., Lewis, T.L., Herrmann, J.E., Blacklow, N.R., Dupuis, K., and Reyes, G.R., 1993, Cloning and characterization of human astrovirus immunoreactive epitopes, J. Virol. 67: 1712–1715.
Marczinke, B., Bloys, A.J., Brown, T.D., Willcocks, M.M., Carter, M.J., and Brierley, I., 1994, The human astrovirus RNA-dependent RNA polymerase coding region is expressed by ribosomal frameshifting, J. Virol. 68: 5588–5595.
Monroe, S.S., Stine, S.E., Gorelkin, L., Herrmann, J.E., Blacklow, N.R., and Glass, R.I., 1991, Temporal synthesis of proteins and RNAs during human astrovirus infection of cultured cells, J. Virol. 65: 641–648.
Monroe, S.S., Jiang, B., Stine, S.E., Koopmans, M., and Glass, R.I., 1993, Subgenomic RNA sequence of human astrovirus supports classification of Astroviridae as a new family of RNA viruses, J. Virol. 67: 3611–3614.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer Science+Business Media New York
About this chapter
Cite this chapter
Gibson, C.A., Chen, J., Monroe, S.A., Denison, M.R. (1998). Expression and Processing of Nonstructural Proteins of the Human Astroviruses. In: Enjuanes, L., Siddell, S.G., Spaan, W. (eds) Coronaviruses and Arteriviruses. Advances in Experimental Medicine and Biology, vol 440. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5331-1_50
Download citation
DOI: https://doi.org/10.1007/978-1-4615-5331-1_50
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-7432-9
Online ISBN: 978-1-4615-5331-1
eBook Packages: Springer Book Archive