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Recent Advances in Riboflavin Biosynthesis

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Flavins and Flavoproteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1146))

Abstract

Riboflavin is biosynthesized from GTP and ribulose 5-phosphate. Whereas the early reactions conducing to 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5′-phosphate show significant taxonomic variation, the subsequent reaction steps are universal in all taxonomic kingdoms. With the exception of a hitherto elusive phosphatase, all enzymes of the pathway have been characterized in some detail at the structural and mechanistic level. Some of the pathway enzymes (GTP cycloyhdrolase II, 3,4-dihydroxy-2-butanone 4-phosphate synthase, riboflavin synthase) have exceptionally complex reaction mechanisms. The commercial production of the vitamin is now entirely based on highly productive fermentation processes. Due to their absence in animals, the pathway enzymes are potential targets for the development of novel anti-infective drugs.

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Acknowledgements

Support by the Deutsche Forschungsgemeinschaft is gratefully acknowledged.

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Authors and Affiliations

  1. Hamburg School of Food Science, Institute of Food Chemistry, University of Hamburg, Grindelallee 117, 20146, Hamburg, Germany

    Ilka Haase, Tobias Gräwert, Boris Illarionov & Markus Fischer

  2. Department of Chemistry, Organic Chemistry & Biochemistry, Technische Universität München, Lichtenbergstraße 4, 85748 Garching, Munich, Germany

    Adelbert Bacher

Authors
  1. Ilka Haase
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  2. Tobias Gräwert
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  3. Boris Illarionov
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  4. Adelbert Bacher
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  5. Markus Fischer
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Corresponding authors

Correspondence to Adelbert Bacher or Markus Fischer .

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Editors and Affiliations

  1. Institut für Physikalische Chemie, Albert-Ludwigs-Universität Freiburg, Germany

    Stefan Weber

  2. Institut für Physikalische Chemie, Albert-Ludwigs-Universität Freiburg, Germany

    Erik Schleicher

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Haase, I., Gräwert, T., Illarionov, B., Bacher, A., Fischer, M. (2014). Recent Advances in Riboflavin Biosynthesis. In: Weber, S., Schleicher, E. (eds) Flavins and Flavoproteins. Methods in Molecular Biology, vol 1146. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0452-5_2

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  • DOI: https://doi.org/10.1007/978-1-4939-0452-5_2

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