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Circulating Cell-Free DNA in Cancer

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Quantitative Real-Time PCR

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1160))

Abstract

This papers deals with the preanalytical and analytical phase of cell-free DNA analysis, highlighting some criticism on sample collection and extraction. We describe a method to accurately quantify total cfDNA in plasma and our particular approach to the measurement of tumor deriving cfDNA.

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Correspondence to Pamela Pinzani .

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© 2014 Springer Science+Business Media New York

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Pinzani, P., Salvianti, F., Orlando, C., Pazzagli, M. (2014). Circulating Cell-Free DNA in Cancer. In: Biassoni, R., Raso, A. (eds) Quantitative Real-Time PCR. Methods in Molecular Biology, vol 1160. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0733-5_13

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  • DOI: https://doi.org/10.1007/978-1-4939-0733-5_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0732-8

  • Online ISBN: 978-1-4939-0733-5

  • eBook Packages: Springer Protocols

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