Abstract
The basis of flow cytometric allergy diagnosis is quantification of changes in expression of basophilic surface membrane markers (Ebo et al., Clin Exp Allergy 34: 332–339, 2004). Upon encountering specific allergens recognized by surface receptor FcεRI-bound IgE, basophils not only secrete and generate quantifiable bioactive mediators but also up-regulate the expression of different markers (e.g., CD63, CD203c) which can be detected by multicolor flow cytometry using specific monoclonal antibodies (Ebo et al., Cytometry B Clin Cytom 74: 201–210, 2008). Here, we describe two flow cytometry-based protocols which allow detection of surface marker activation (Method 1) and changes in intragranular histamine (Method 2), both reflecting different facets of basophil activation.
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References
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Bridts, C.H., Sabato, V., Mertens, C., Hagendorens, M.M., De Clerck, L.S., Ebo, D.G. (2014). Flow Cytometric Allergy Diagnosis: Basophil Activation Techniques. In: Gibbs, B., Falcone, F. (eds) Basophils and Mast Cells. Methods in Molecular Biology, vol 1192. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1173-8_11
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DOI: https://doi.org/10.1007/978-1-4939-1173-8_11
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-1173-8
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