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Perspectives in Glycomics and Lectin Engineering

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Lectins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1200))

Abstract

This chapter would like to provide a short survey of the most promising concepts applied recently in analysis of glycoproteins based on lectins. The first part describes the most exciting analytical approaches used in the field of glycoprofiling based on integration of nanoparticles, nanowires, nanotubes, or nanochannels or using novel transducing platforms allowing to detect very low levels of glycoproteins in a label-free mode of operation. The second part describes application of recombinant lectins containing several tags applied for oriented and ordered immobilization of lectins. Besides already established concepts of glycoprofiling several novel aspects, which we think will be taken into account for future, more robust glycan analysis, are described including modified lectins, peptide lectin aptamers, and DNA aptamers with lectin-like specificity introduced by modified nucleotides. The last part of the chapter describes a novel concept of a glycocodon, which can lead to a better understanding of glycan–lectin interaction and for design of novel lectins with unknown specificities and/or better affinities toward glycan target or for rational design of peptide lectin aptamers or DNA aptamers.

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Acknowledgement

The financial support from the Slovak research and development agency APVV 0282-11, from VEGA 2/0127/10 and 2/0162/14 is acknowledged. The research leading to these results has received partly funding from the European Research Council under the European Union’s Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement n. 311532, from the European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant agreement n. 317420 and from Qatar Foundation under Project n. 6-381-1-078. This contribution was partly supported by the project: Centre of excellence for white-green biotechnology, ITMS 26220120054, supported by the Research & Development Operational Programme funded by the ERDF.

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Authors and Affiliations

  1. Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dubravska Cesta 9, 845 38, Bratislava, Slovakia

    Jan Tkac, Tomas Bertok, Jozef Nahalka & Peter Gemeiner

  2. Institute of Chemistry, Center of Excellence for White-Green Biotechnology, Slovak Academy of Sciences, Trieda Andreja Hlinku 2, SK-94976, Nitra, Slovakia

    Jan Tkac & Jozef Nahalka

Authors
  1. Jan Tkac
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  2. Tomas Bertok
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  3. Jozef Nahalka
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  4. Peter Gemeiner
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Correspondence to Peter Gemeiner .

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  1. National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan

    Jun Hirabayashi

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Tkac, J., Bertok, T., Nahalka, J., Gemeiner, P. (2014). Perspectives in Glycomics and Lectin Engineering. In: Hirabayashi, J. (eds) Lectins. Methods in Molecular Biology, vol 1200. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1292-6_37

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  • DOI: https://doi.org/10.1007/978-1-4939-1292-6_37

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1291-9

  • Online ISBN: 978-1-4939-1292-6

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