Abstract
The aim of the study was to assess the link between the metabolic profile and the proliferation capacity of a range of human and murine cancer cell lines. First, the combination of mitochondrial respiration and glycolytic efficiency measurements allowed the determination of different metabolic profiles among the cell lines, ranging from a mostly oxidative to a mostly glycolytic phenotype. Second, the study revealed that cell proliferation, evaluated by DNA synthesis measurements, was statistically correlated to glycolytic efficiency. This indicated that glycolysis is the key energetic pathway linked to cell proliferation rate. Third, to validate this hypothesis and exclude non-metabolic factors, mitochondria-depleted were compared to wild-type cancer cells, and the data showed that enhanced glycolysis observed in mitochondria-depleted cells is also associated with an increase in proliferation capacity.
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Acknowledgments
This work was supported by grants from the Télévie, the Belgian National Fund for Scientific Research (F.R.S.-FNRS), the Fonds Joseph Maisin, the Fondation Belge contre le Cancer, the Saint-Luc Foundation, the Actions de Recherches Concertées-Communauté Française de Belgique (ARC 04/09-317), and the European Research Council (FP7/2007-2013 ERC Independent Researcher Starting Grant No. 243188 TUMETABO). B.F.J. and P.S. are Research Associates and P.D. a Research Fellow of the F.R.S.-FNRS. G.D. is a Télévie Research Fellow.
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De Preter, G. et al. (2016). Direct Evidence of the Link Between Energetic Metabolism and Proliferation Capacity of Cancer Cells In Vitro. In: Elwell, C.E., Leung, T.S., Harrison, D.K. (eds) Oxygen Transport to Tissue XXXVII. Advances in Experimental Medicine and Biology, vol 876. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-3023-4_26
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DOI: https://doi.org/10.1007/978-1-4939-3023-4_26
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