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Infectious Viral Quantification of Chikungunya Virus—Virus Plaque Assay

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Chikungunya Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1426))

Abstract

The plaque assay is an essential method for quantification of infectious virus titer. Cells infected with virus particles are overlaid with a viscous substrate. A suitable incubation period results in the formation of plaques, which can be fixed and stained for visualization. Here, we describe a method for measuring Chikungunya virus (CHIKV) titers via virus plaque assays.

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Correspondence to Justin Jang Hann Chu .

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© 2016 Springer Science+Business Media New York

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Kaur, P., Lee, R.C.H., Chu, J.J.H. (2016). Infectious Viral Quantification of Chikungunya Virus—Virus Plaque Assay. In: Chu, J., Ang, S. (eds) Chikungunya Virus. Methods in Molecular Biology, vol 1426. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3618-2_9

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  • DOI: https://doi.org/10.1007/978-1-4939-3618-2_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3616-8

  • Online ISBN: 978-1-4939-3618-2

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