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Characterization of Cancer Stem Cells

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Tumor Angiogenesis Assays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1464))

Abstract

There is biological and clinical evidence that mammalian tumors are highly heterogeneous at single-cell level, and that only a minority of the cancer cells have limitless replicative potential in vitro and in vivo. Here we review the different strategies currently used for the functional isolation of cancer cells with in vivo cancer initiation potential and self-renewal. These tools are currently used to better define their molecular, phenotypic, and drug-resistance characteristics in preclinical and clinical studies.

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Acknowledgments

Supported in part by AIRC (Associazione Italiana per la Ricerca sul Cancro), Fondazione Umberto Veronesi, and Ministero della Salute.

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Authors and Affiliations

  1. Laboratory of Hematology–Oncology, European Institute of Oncology, Via Ripamonti 435, 20141, Milan, Italy

    Stefania Orecchioni & Francesco Bertolini

Authors
  1. Stefania Orecchioni
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  2. Francesco Bertolini
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Corresponding author

Correspondence to Francesco Bertolini .

Editor information

Editors and Affiliations

  1. Neurosciences and Sensory Organs, University of Bari,School of Med Dept of Basic Med Sci, Bari, Italy

    Domenico Ribatti

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Orecchioni, S., Bertolini, F. (2016). Characterization of Cancer Stem Cells. In: Ribatti, D. (eds) Tumor Angiogenesis Assays. Methods in Molecular Biology, vol 1464. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3999-2_5

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  • DOI: https://doi.org/10.1007/978-1-4939-3999-2_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3997-8

  • Online ISBN: 978-1-4939-3999-2

  • eBook Packages: Springer Protocols

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