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Methods to Analyze EVs

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Exosomes and Microvesicles

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1545))

Abstract

Research in the field of extracellular vesicles (EVs) is challenged by the small size of the nano-sized particles. Apart from the use of transmission and scanning electron microscopy, established technical platforms to visualize, quantify, and characterize nano-sized EVs were lacking. Recently, methodologies to characterize nano-sized EVs have been developed. This chapter aims to summarize physical principles of novel and conventional technologies to be used in the EV field and to discuss advantages and limitations.

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Authors and Affiliations

  1. Institute for Transfusion Medicine, University Hospital Essen, University Duisburg-Essen, Virchowstr. 179, 45147, Essen, Germany

    Bernd Giebel

  2. Particle Metrix GmbH, Am Latumer See 13, 40668, Meerbusch, Germany

    Clemens Helmbrecht

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  1. Bernd Giebel
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  2. Clemens Helmbrecht
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Correspondence to Bernd Giebel .

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Editors and Affiliations

  1. Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Victoria, Australia

    Andrew F Hill

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Giebel, B., Helmbrecht, C. (2017). Methods to Analyze EVs. In: Hill, A. (eds) Exosomes and Microvesicles. Methods in Molecular Biology, vol 1545. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6728-5_1

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  • DOI: https://doi.org/10.1007/978-1-4939-6728-5_1

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