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Chapter 13 Human Ovarian Tissue Vitrification

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Cryopreservation of Mammalian Gametes and Embryos

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1568))

Abstract

Ovarian freezing and transplantation has garnered increasing interest as a potential way of preserving fertility in cancer patients as well as for women who just wish to delay childbearing. This chapter spells out our techniques of ovarian cortex vitrification and results for frozen compared to fresh ovarian cortex transplantation (in one single series from one center for the sake of consistency), as well as potentially provides insight into the mechanism behind ovarian follicle recruitment. This represents an effort to simplify and popularize an approach that has yielded favorable results (all cases recovered ovulation and 75% had successful spontaneous pregnancy) in one single, disciplined study. It should be clear that this is a review for the more general reader of our original scientific papers published in Reproductive BioMedicine Online, New England Journal of Medicine, Fertility and Sterility, Human Reproduction, Molecular Human Reproduction, and Journal of Assisted Reproduction and Genetics (JARG).

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Author information

Authors and Affiliations

  1. Infertility Center of St. Louis, 224 South Woods Mill Road, Suite 730, St. Louis, MO, 63017, USA

    Sherman Silber M.D.

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  1. Sherman Silber M.D.
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Correspondence to Sherman Silber M.D. .

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Editors and Affiliations

  1. Reproductive Biology Associates, Atlanta, Georgia, USA

    Zsolt Peter Nagy

  2. ASTRA Fertility Group, Mississauga, Ontario, Canada

    Alex C. Varghese

  3. Cleveland Clinic Foundation, Cleveland, Ohio, USA

    Ashok Agarwal

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Silber, S. (2017). Chapter 13 Human Ovarian Tissue Vitrification. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) Cryopreservation of Mammalian Gametes and Embryos. Methods in Molecular Biology, vol 1568. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6828-2_13

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  • DOI: https://doi.org/10.1007/978-1-4939-6828-2_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6826-8

  • Online ISBN: 978-1-4939-6828-2

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