Abstract
Transplanted induced pluripotent stem cells (IPSC ) and embryonic stem cells (ESC) exhibit enhanced survival in ischemic tissues and promote survival of neighboring cells via paracrine effects. Recent studies indicate that stem cells can secrete extracellular vesicles (EV), which can shuttle noncoding RNA between cells and facilitate intercellular signaling and communication between donor stem cells and recipient tissues. Direct transplantation of IPSC -derived EV (IPSC -EV) is highly effective at promoting survival and preventing apoptosis of cardiomyocytes in a mouse model of acute myocardial ischemia–reperfusion (MI/R). Here, we describe a feasible protocol to purify EV from cultured IPSC .
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References
Phillips MI et al (2002) Vigilant vector: heart-specific promoter in an adeno-associated virus vector for cardioprotection. Hypertension 39(2 Pt 2):651–655
Phillips MI, Tang YL, Pinkernell K (2008) Stem cell therapy for heart failure: the science and current progress. Futur Cardiol 4(3):285–298
Tang YL et al (2005) Paracrine action enhances the effects of autologous mesenchymal stem cell transplantation on vascular regeneration in rat model of myocardial infarction. Ann Thorac Surg 80(1):229–236. discussion 236–7
Tang YL et al (2004) Autologous mesenchymal stem cell transplantation induce VEGF and neovascularization in ischemic myocardium. Regul Pept 117(1):3–10
Pan A, Weintraub NL, Tang Y (2014) Enhancing stem cell survival in an ischemic heart by CRISPR-dCas9-based gene regulation. Med Hypotheses 83(6):702–705
Chen L et al (2013) Two-step protocol for isolation and culture of cardiospheres. Methods Mol Biol 1036:75–80
Tang YL et al (2013) Cardiac-derived stem cell-based therapy for heart failure: progress and clinical applications. Exp Biol Med (Maywood) 238(3):294–300
Chen L et al (2013) Cardiac progenitor-derived Exosomes protect ischemic myocardium from acute ischemia/reperfusion injury. Biochem Biophys Res Commun 431(3):566–571
Wang Y et al (2015) Exosomes/microvesicles from induced pluripotent stem cells deliver cardioprotective miRNAs and prevent cardiomyocyte apoptosis in the ischemic myocardium. Int J Cardiol 192:61–69
Zhang L et al (2014) MiR-92a regulates viability and angiogenesis of endothelial cells under oxidative stress. Biochem Biophys Res Commun 446(4):952–958
Zhang L et al (2014) miR-92a inhibits vascular smooth muscle cell apoptosis: role of the MKK4-JNK pathway. Apoptosis 19(6):975–983
Tang Y et al (2015) MicroRNA-150 protects the mouse heart from ischaemic injury by regulating cell death. Cardiovasc Res 106(3):387–397
Tang Y et al (2012) Cross talk between the Notch signaling and noncoding RNA on the fate of stem cells. Prog Mol Biol Transl Sci 111:175–193
Archer K et al (2015) Long non-coding RNAs as master regulators in cardiovascular diseases. Int J Mol Sci 16(10):23651–23667
Bayoumi AS et al (2016) Crosstalk between long noncoding RNAs and microRNAs in health and disease. Int J Mol Sci 17(3):356
Khan M et al (2015) Embryonic stem cell-derived exosomes promote endogenous repair mechanisms and enhance cardiac function following myocardial infarction. Circ Res 117(1):52–64
Sahoo S et al (2011) Exosomes from human CD34(+) stem cells mediate their proangiogenic paracrine activity. Circ Res 109(7):724–728
Ansa-Addo EA et al (2010) Human plasma membrane-derived vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cells. J Immunol 185(9):5236–5246
Ostolska I, Wisniewska M (2014) Application of the zeta potential measurements to explanation of colloidal CrO stability mechanism in the presence of the ionic polyamino acids. Colloid Polym Sci 292(10):2453–2464
Acknowledgment
This work was supported NIH grants AR070029 and HL086555 (to Y.T.), and HL076684 and HL62984 (to N.L.W.).
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Chen, Z. et al. (2017). Isolation of Extracellular Vesicles from Stem Cells. In: Kuo, W., Jia, S. (eds) Extracellular Vesicles. Methods in Molecular Biology, vol 1660. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7253-1_32
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DOI: https://doi.org/10.1007/978-1-4939-7253-1_32
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