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Evaluating Thyroid Disrupting Chemicals In Vivo Using Xenopus laevis

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Thyroid Hormone Nuclear Receptor

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1801))

Abstract

Using in vivo animal model systems for chemical screening can permit evaluation of the signaling pathways implicated. Xenopus laevis is an ideal model organism to test thyroid axis disruption as thyroid hormones are highly conserved across vertebrates. Here, we describe a high-throughput assay using non-feeding embryonic stage transgenic X. laevis (TH/bZip) to screen for thyroid disrupting chemicals using a 3 day exposure protocol. We further describe a protocol to detect endocrine disruption of thyroid axis by the analysis of gene expression using wild-type X. laevis.

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Notes

  1. 1.

    European commission study 2006. “Environment fact sheet: REACH - a new chemicals policy for the EU”. Consulted on 21st July 2017 - http://ec.europa.eu/environment/chemicals/reach/pdf/reach.pdf

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Acknowledgments

We thank Gérard Benisti, Philippe Durand and Jean-Paul Chaumeil for excellent animal care and thank Sébastien Le Mével for his input in the methods which are routinely used. This protocol has been refined thanks to work supported by grants from Centre National de la Recherche Scientifique (CNRS), Muséum National d’Histoire Naturelle (MNHN), and from European Union DevCom FP7-People-2013-ITN N°607142.

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Authors and Affiliations

  1. Evolution des Régulations Endocriniennes, Département “Adaptation du Vivant”, UMR 7221 Muséum National d’Histoire Naturelle /CNRS, 7 rue Cuvier, Paris, 75005, France

    Bilal B. Mughal, Barbara A. Demeneix & Jean-Baptiste Fini

Authors
  1. Bilal B. Mughal
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  2. Barbara A. Demeneix
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  3. Jean-Baptiste Fini
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Corresponding author

Correspondence to Barbara A. Demeneix .

Editor information

Editors and Affiliations

  1. Centre Léon Bérard, Cancer Research Center of Lyon, Lyon, France

    Michelina Plateroti

  2. École normale supérieure de Lyon, Lyon, France

    Jacques Samarut

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Mughal, B.B., Demeneix, B.A., Fini, JB. (2018). Evaluating Thyroid Disrupting Chemicals In Vivo Using Xenopus laevis . In: Plateroti, M., Samarut, J. (eds) Thyroid Hormone Nuclear Receptor. Methods in Molecular Biology, vol 1801. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7902-8_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7902-8_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7901-1

  • Online ISBN: 978-1-4939-7902-8

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