Abstract
Activation of signal transducer and activator of transcription 6 (STAT6) is a key signaling pathway in macrophage function, and is required for the so-called alternative (M2) activation of macrophages. Interleukin (IL)-4 and IL-13 are important M2 polarizing cytokines that act through STAT6 by inducing its phosphorylation and promoting transcription of STAT6-responsive genes. Inactivation of STAT6 signaling in macrophages has not been fully explored; however, a recent model suggests that inactivation of STAT6 signaling can occur via ubiquitination and proteasomal degradation. In this chapter, we describe a combination of techniques that can be used to study the activation/inactivation of STAT6 signaling in macrophages.
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Acknowledgments
The work in the authors’ laboratory is supported by a German Research Fund (DFG) grant, by the European Foundation for the Study of Diabetes (EFSD) and by CME University of Ulm (Director Prof. Dr. Jan Tuckermann). SFHW is supported by the German Academic Exchange Service (DAAD). Scopus author identifiers of the authors are 57194705909 (S.F.H.W.) and 7801656864 (T.R.).
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Waqas, S.F.H., Ampem, G., Röszer, T. (2019). Analysis of IL-4/STAT6 Signaling in Macrophages. In: Badr, M. (eds) Nuclear Receptors. Methods in Molecular Biology, vol 1966. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9195-2_17
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DOI: https://doi.org/10.1007/978-1-4939-9195-2_17
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