Abstract
High-throughput sequencing (HTS) is a powerful tool employed by plant virologists for the detection of viruses, the characterization of virus genomes and the study of host-pathogen interactions. Virus detection has been an important application of this technology, which has resulted in the discovery of novel viruses or viral strains as well as for the detection of known viruses in a plant sample. Here we describe the entire process that needs to be considered for the genome analysis of Citrus tristeza virus (CTV) by HTS, including the experimental design, sample preparation, nucleic acid purification, HTS library construction, and bioinformatic analysis.
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Acknowledgments
The authors would like to acknowledge Citrus Research International (CRI), Instituto Nacional de Investigaciones Agrarias (INIA), and Instituto Valenciano de Investigaciones Agrarias (IVIA) for sample collection and project funding. The financial assistance of the National Research Foundation (NRF) toward this research is hereby acknowledged. The opinions expressed and conclusions arrived at are those of the authors and are not necessarily to be attributed to the NRF, INIA, IVIA, or CRI.
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Ruiz-García, A.B., Bester, R., Olmos, A., Maree, H.J. (2019). Bioinformatic Tools and Genome Analysis of Citrus tristeza virus. In: Catara, A., Bar-Joseph, M., Licciardello, G. (eds) Citrus Tristeza Virus. Methods in Molecular Biology, vol 2015. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9558-5_12
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DOI: https://doi.org/10.1007/978-1-4939-9558-5_12
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