Abstract
Natural Killer (NK) cells are cells of the innate immune system with characteristic effector functions, including recognition and lysis of virus-infected or tumor cells and production of immunoregulatory cytokines, particularly interferon-gamma (IFN-γ). NK cells account for between 10 and 15% of peripheral blood lymphocytes and are also present in synovial fluid and tissue where they might potentially contribute to amplification of the inflammatory process through interactions with macrophages and dendritic cells. This chapter outlines methods of assessing the phenotype of NK cells through analysis of NK-cell markers and the function of NK cells through cytotoxicity assays and measurement of cytokine production.
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References
Cooper, M. A., Fehniger, T. A., and Caligiuri, M. A. (2001) The biology of human natural killer-cell subsets. Trends. Immunol. 22, 633–640.
McQueen, K. L. and Parham, P. (2002) Variable receptors controlling activation and inhibition of NK cells. Curr. Opin. Immunol. 14, 615–621.
Cooper, M. A., Fehniger, T. A., Turner, S. C., et al. (2001) Human natural killer cells: a unique innate immunoregulatory role for the CD56(bright) subset. Blood 97, 3146–3151.
Jacobs, R., Hintzen, G., Kemper, A., et al. (2001) CD56bright cells differ in their KIR repertoire and cytotoxic features from CD56dim NK cells. Eur. J. Immunol. 31, 3121–3127.
Campbell, J. J., Qin, S., Unutmaz, D., et al. (2001). Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire. J. Immunol. 166, 6477–6482.
Fehniger, T. A., Cooper, M. A., Nuovo, G. J., et al. (2003) CD56bright natural killer cells are present in human lymph nodes and are activated by T cell-derived IL-2: a potential new link between adaptive and innate immunity. Blood 101, 3052–3057.
Dalbeth, N. and Callan, M. F. (2002) A subset of natural killer cells is greatly expanded within inflamed joints. Arthritis Rheum. 46, 1763–1772.
Kaufmann, O., Georgi, T., and Dietel, M. (1997). Utility of 123C3 monoclonal antibody against CD56 (NCAM) for the diagnosis of small cell carcinomas on paraffin sections. Hum. Pathol. 28, 1373–1378.
Mulder, W. M., Koenen, H., van de Muysenberg, A. J., Bloemena, E., Wagstaff, J., and Scheper, R. J. (1994) Reduced expression of distinct T-cell CD molecules by collagenase/DNase treatment. Cancer Immunol. Immunother. 38, 253–258.
Uhrberg, M., Valiante, N. M., Shum, B. P., et al. (1997) Human diversity in killer cell inhibitory receptor genes. Immunity 7, 753–763.
Ferlazzo, G., Tsang, M. L., Moretta, L., Melioli, G., Steinman, R. M., and Munz, C. (2002) Human dendritic cells activate resting natural killer (NK) cells and are recognized via the NKp30 receptor by activated NK cells. J. Exp. Med. 195, 343–351.
Gerosa, F., Baldani-Guerra, B., Nisii, C., Marchesini, V., Carra, G., and Trinchieri, G. (2002) Reciprocal activating interaction between natural killer cells and dendritic cells. J. Exp. Med. 195, 327–333.
Piccioli, D., Sbrana, S., Melandri, E., and Valiante, N. M. (2002) Contact-dependent stimulation and inhibition of dendritic cells by natural killer cells. J. Exp. Med. 195, 335–341.
Pala, P., Hussell, T., and Openshaw, P. J. (2000) Flow cytometric measurement of intracellular cytokines. J. Immunol. Methods 243, 107–124.
Domzig, W., Stadler, B. M., and Herberman, R. B. (1983) Interleukin 2 dependence of human natural killer (NK) cell activity. J. Immunol. 130, 1970–1973.
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© 2007 Humana Press Inc., Totowa, NJ
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Dalbeth, N., Callan, M.F.C. (2007). Phenotypic and Functional Analysis of Synovial Natural Killer Cells. In: Cope, A.P. (eds) Arthritis Research. Methods in Molecular Medicine, vol 136. Humana Press. https://doi.org/10.1007/978-1-59745-402-5_11
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DOI: https://doi.org/10.1007/978-1-59745-402-5_11
Publisher Name: Humana Press
Print ISBN: 978-1-58829-918-5
Online ISBN: 978-1-59745-402-5
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