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Microarrays for the Study of Viral Gene Expression During Human Cytomegalovirus Latent Infection

  • Protocol
Clinical Bioinformatics

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 141))

Summary

Human cytomegalovirus (HCMV) is one of the largest known DNA viruses. It is ubiquitous, and following resolution of primary productive infection, it persists in the human host by establishing a lifelong latent infection in myeloid lineage cells such as monocytes and their progenitors. Most adults with HCMV infection are healthy but it can cause neurologic deficits in infants, and remains an important cause of morbidity and mortality in the immunosuppressed patient. Microarray-based studies of HCMV have provided useful information about genes that are transcriptionally active during both productive and latent phases of infection. This chapter describes how to study genes in HCMV using microarrays and two cell types (productively infected human foreskin fibroblasts, and latently infected primary human myeloid progenitor cells).

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Abbreviations

aRNA:

amplified RNA

HCMV:

human cytomegalovirus

HFF:

human foreskin fibroblast

ORF:

open reading frame

RT-PCR:

reverse transcriptase PCR

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Acknowledgments

The authors would like to thank Chris Bye and Karen Bythe for their assistance with microarray data analyses.

Author information

Authors and Affiliations

  1. Centre for Virus Research, Westmead Millennium Institute, Westmead, New South Wales, Australia

    Barry Slobedman & Allen K. L. Cheung

Authors
  1. Barry Slobedman
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  2. Allen K. L. Cheung
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Editor information

Editors and Affiliations

  1. Department of Molecular and Clinical Genetics, University of Sydney Central Clinical School Royal Prince Alfred Hospital, Camperdown, New South Wales, Australia

    Ronald J.A. Trent

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Slobedman, B., Cheung, A.K.L. (2008). Microarrays for the Study of Viral Gene Expression During Human Cytomegalovirus Latent Infection. In: Trent, R.J. (eds) Clinical Bioinformatics. Methods in Molecular Medicine™, vol 141. Humana Press. https://doi.org/10.1007/978-1-60327-148-6_9

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  • DOI: https://doi.org/10.1007/978-1-60327-148-6_9

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-791-4

  • Online ISBN: 978-1-60327-148-6

  • eBook Packages: Springer Protocols

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