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Osteoclast Formation in Mouse Co-cultures

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Bone Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 816))

Abstract

The murine co-culture assay is used to generate mature osteoclasts from bone marrow precursors by culturing them with osteoblasts that are stimulated with 1,25-dihydroxy vitamin D3 and prostaglandin E2. This assay is used particularly to analyse osteoblast–osteoclast interactions and to determine the cell type affected in knock-out or transgenic mice. This chapter describes also the isolation of bone marrow cells from mice and the methods to purify and replate mature osteoclasts.

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Author information

Authors and Affiliations

  1. Musculoskeletal Research Programme, Division of Applied Medicine, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK

    Cecile Itzstein

  2. Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK

    Robert J. van ‘t Hof

Authors
  1. Cecile Itzstein
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  2. Robert J. van ‘t Hof
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Corresponding author

Correspondence to Robert J. van ‘t Hof .

Editor information

Editors and Affiliations

  1. Institute of Medical Sciences, University of Aberdeen, Forester Hill, Aberdeen, AB25 2ZD, United Kingdom

    Miep H. Helfrich

  2. Institute of Genetics and Molecular Medi, Rheumatic Diseases Unit, University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XU, United Kingdom

    Stuart H. Ralston

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Itzstein, C., van ‘t Hof, R.J. (2012). Osteoclast Formation in Mouse Co-cultures. In: Helfrich, M., Ralston, S. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 816. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-415-5_12

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  • DOI: https://doi.org/10.1007/978-1-61779-415-5_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-414-8

  • Online ISBN: 978-1-61779-415-5

  • eBook Packages: Springer Protocols

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