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Plant Genetics for Forensic Applications

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Plant DNA Fingerprinting and Barcoding

Part of the book series: Methods in Molecular Biology ((MIMB,volume 862))

Abstract

An emerging application for plant DNA fingerprinting and barcoding involves forensic investigations. Examples of DNA analysis of botanical evidence include crime scene analysis, identifying the source of commercial plant products, and investigation of trade in illicit drugs. Here, we review real and potential applications of DNA-based forensic botany and provide a protocol for microsatellite genotyping of leaf material, a protocol that could be used to link a suspect to a victim or to a crime scene.

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References

  1. Rubinoff, D., Cameron, S., and Will, K. (2006) Are plant DNA barcodes a search for the Holy Grail?, Trends in Ecology & Evolution 21, 1–2.

    Article  Google Scholar 

  2. Taberlet, P., Coissac, E., Pompanon, F., Gielly, L., Miquel, C., Valentini, A., Vermat, T., Corthier, G., Brochmann, C., and Willerslev, E. (2007) Power and limitations of the chloroplast trnL (UAA) intron for plant DNA barcoding, Nucleic Acids Research 35.

    Google Scholar 

  3. Ferri, G., Alu, M., Corradini, B., and Beduschi, G. (2009) Forensic botany: species identification of botanical trace evidence using a multigene barcoding approach, International Journal of Legal Medicine 123, 395–401.

    Article  PubMed  Google Scholar 

  4. Ward, J., Gilmore, S. R., Robertson, J., and Peakall, R. (2009) A grass molecular identification system for forensic botany: A critical evaluation of the strengths and limitations, Journal of Forensic Sciences 54, 1254–1260.

    Article  PubMed  CAS  Google Scholar 

  5. Mestel, R. (1993) Murder trial features trees genetic fingerprint, New Scientist 138, 6.

    Google Scholar 

  6. Yoon, C. K. (1993) Forensic Science - Botanical witness for the prosecution, Science 260, 894–895.

    Article  PubMed  CAS  Google Scholar 

  7. Korpelainen, H., and Virtanen, V. (2003) DNA fingerprinting of mosses, Journal of forensic sciences 48, 804–807.

    PubMed  CAS  Google Scholar 

  8. Craft, K. J., Owens, J. D., and Ashley, M. V. (2007) Application of plant DNA markers in forensic botany: Genetic comparison of Quercus evidence leaves to crime scene trees using microsatellites, Forensic Science International 165, 64–70.

    Article  PubMed  CAS  Google Scholar 

  9. Lee, C. L., Miller Coyle, H., Carita, E., Ladd, C., Yang, N. C. S., Palmbach, T. M., Hsu, I. C., and Lee, H. C. (2006) DNA analysis of digested tomato seeds in stomach contents, American Journal of Forensic Medicine and Pathology 27, 121–125.

    Article  PubMed  Google Scholar 

  10. Lee, C. L., Miller Coyle, H., and Lee, H. C. (2007) Genetic analysis of individual seeds by amplified fragment length polymorphism method, Croatian Medical Journal 48, 563–565.

    PubMed  CAS  Google Scholar 

  11. Lee, E. J., Kim, S. C., Hwang, I. K., Yang, H. J., Kim, Y. S., Han, M. S., Yang, M. S., and Lee, Y. H. (2009) The identification of ingested dandelion juice in gastric contents of a deceased person by direct sequencing and GC-MS methods, Journal of Forensic Sciences 54, 721–727.

    Article  PubMed  CAS  Google Scholar 

  12. Techen, N., Crockett, S. L., Khan, I. A., and Scheffler, B. E. (2004) Authentication of medicinal plants using molecular biology techniques to compliment conventional methods, Current Medicinal Chemistry 11, 1391–1401.

    PubMed  CAS  Google Scholar 

  13. Rachmayanti, Y., Leinemann, L., Gailing, O., and Finkeldey, R. (2009) DNA from processed and unprocessed wood: Factors influencing the isolation success, Forensic Science International-Genetics 3, 185–192.

    Article  PubMed  CAS  Google Scholar 

  14. Joshi, K., Chavan, P., Warude, D., and Patwardhan, B. (2004) Molecular markers in herbal drug technology, Current Science 87, 159–165.

    CAS  Google Scholar 

  15. Yip, P., Chau, C., Mak, C., and Kwan, H. (2007) DNA methods for identification of Chinese medicinal materials, Chinese Medicine 2, 9.

    Article  PubMed  Google Scholar 

  16. Zhang, Y. B., Shaw, P. C., Sze, C. W., Wang, Z. T., and Tong, Y. (2007) Molecular authentication of Chinese herbal materials, Journal of Food and Drug Analysis 15, 1–9.

    Google Scholar 

  17. Sucher, N. J., and Carles, M. C. (2008) Genome-based approaches to the authentication of medicinal plants, Planta Medica 74, 603–623.

    Article  PubMed  CAS  Google Scholar 

  18. Jiang, Y., David, B., Tu, P. F., and Barbin, Y. (2010) Recent analytical approaches in quality control of traditional Chinese medicines-A review, Analytica Chimica Acta 657, 9–18.

    Article  PubMed  CAS  Google Scholar 

  19. Mihalov, J. J., Marderosian, A. D., and Pierce, J. C. (2000) DNA identification of commercial ginseng samples, Journal of Agricultural and Food Chemistry 48, 3744–3752.

    Article  PubMed  CAS  Google Scholar 

  20. Fushimi, H., Komatsu, K., Isobe, M., and Namba, T. (1996) 18 S ribosomal RNA gene sequences of three Panax species and the corresponding Ginseng drugs, Biological & Pharmaceutical Bulletin 19, 1530–1532.

    Article  CAS  Google Scholar 

  21. Qin, J. H., Leung, F. C., Fung, Y. S., Zhu, D. R., and Lin, B. C. (2005) Rapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection, Analytical and Bioanalytical Chemistry 381, 812–819.

    Article  PubMed  CAS  Google Scholar 

  22. Ha, W. Y., Shaw, P. C., Liu, J., Yau, F. C. F., and Wang, J. (2002) Authentication of Panax ginseng and Panax quinquefolius using amplified fragment length polymorphism (AFLP) and directed amplification of minisatellite region DNA (DAMD), Journal of Agricultural and Food Chemistry 50, 1871–1875.

    Article  PubMed  CAS  Google Scholar 

  23. Del Serrone, P., Attorri, L., Gallinella, B., Gallo, F. R., Federici, E., and Palazzino, G. (2006) Molecular identification of Panax ginseng C.A. Meyer in ginseng commercial products, Natural Product Communications 1, 1137–1140.

    Google Scholar 

  24. Lau, D. T. W., Shaw, P. C., Wang, J., and But, P. P. H. (2001) Authentication of medicinal Dendrobium species by the internal transcribed spacer of ribosomal DNA, Planta Medica 67, 456–460.

    Article  PubMed  CAS  Google Scholar 

  25. Ding, X. Y., Wang, Z. T., Zhou, K. Y., Xu, L. S., Xu, H., and Wang, Y. Q. (2003) Allele-specific primers for diagnostic PCR authentication of Dendrobium officinale, Planta Medica 69, 587–588.

    Article  PubMed  CAS  Google Scholar 

  26. Zhang, Y. B., Wang, J., Wang, Z. T., But, P. P. H., and Shaw, P. C. (2003) DNA microarray for identification of the herb of Dendrobium species from Chinese medicinal formulations, Planta Medica 69, 1172–1174.

    Article  PubMed  CAS  Google Scholar 

  27. Xu, H., Wang, Z. T., Ding, X. Y., Zhou, K. Y., and Xu, L. S. (2006) Differentiation of Dendrobium species used as “Huangcao Shihu” by rDNA ITS sequence analysis, Planta Medica 72, 89–92.

    Article  PubMed  CAS  Google Scholar 

  28. Sze, S. C. W., Zhang, K. Y. B., Shaw, P. C., But, P. P. H., Ng, T. B., and Tong, Y. (2008) A DNA microarray for differentiation of the Chinese medicinal herb Dendrobium officinale (Fengdou Shihu) by its 5 S ribosomal DNA intergenic spacer region, Biotechnology and Applied Biochemistry 49, 149–154.

    Article  PubMed  CAS  Google Scholar 

  29. Feng, T., Liu, S., and He, X. J. (2010) Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA, Electronic Journal of Biotechnology 13.

    Google Scholar 

  30. Ma, X. Q., Duan, J. A., Zhu, D. Y., Dong, T. T. X., and Tsim, K. W. K. (2000) Species identification of Radix Astragali (Huangqi) by DNA sequence of its 5 S-rRNA spacer domain, Phytochemistry 54, 363–368.

    Article  PubMed  CAS  Google Scholar 

  31. Guo, H. Y., Wang, W. W., Yang, N., Guo, B. L., Zhang, S., Yang, R. J., Yuan, Y., Yu, J. L., Hu, S. N., Sun, Q. S., and Yu, J. (2010) DNA barcoding provides distinction between Radix Astragali and its adulterants, Science China-Life Sciences 53, 992–999.

    Article  PubMed  CAS  Google Scholar 

  32. Zerega, N. J. C., Mori, S., Lindqvist, C., Zheng, Q. Y., and Motley, T. J. (2002) Using amplified fragment length polymorphisms (AFLP) to identify black cohosh (Actaea racemosa), Economic Botany 56, 154–164.

    Article  CAS  Google Scholar 

  33. Wang, C. Z., Li, P., Ding, J. Y., Peng, X., and Yuan, C. S. (2007) Simultaneous identification of Bulbus Fritillariae cirrhosae using PCR-RFLP analysis, Phytomedicine 14, 628–632.

    Article  PubMed  CAS  Google Scholar 

  34. Yang, Z. Y., Chao, Z., Huo, K. K., Xie, H., Tian, Z. P., and Pan, S. L. (2007) ITS sequence analysis used for molecular identification of the Bupleurum species from northwestern China, Phytomedicine 14, 416–422.

    Article  PubMed  CAS  Google Scholar 

  35. Lin, W. Y., Chen, L. R., and Lin, T. Y. (2008) Rapid authentication of Bupleurum species using an array of immobilized sequence-specific oligonucleotide probes, Planta Medica 74, 464–469.

    Article  PubMed  CAS  Google Scholar 

  36. Xue, H. G., Zhou, S. D., He, X. J., and Yu, Y. (2007) Molecular authentication of the traditional Chinese medicinal plant Euphorbia pekinensis, Planta Medica 73, 91–93.

    Article  PubMed  CAS  Google Scholar 

  37. Xue, C. Y., Li, D. Z., and Wang, Q. Z. (2008) Identification and quantification of the ­traditional Chinese medicinal plant Gentiana macrophylla using Taqman Real-Time PCR, Planta Medica 74, 1842–1845.

    Article  PubMed  CAS  Google Scholar 

  38. LeRoy, A., Potter, E., Woo, H. H., Heber, D., and Hirsch, A. M. (2002) Characterization and identification of alfalfa and red clover dietary supplements using a PCR-based method, Journal of Agricultural and Food Chemistry 50, 5063–5069.

    Article  PubMed  CAS  Google Scholar 

  39. Manissorn, J., Sukrong, S., Ruangrungsi, N., and Mizukami, H. (2010) Molecular Phylogenetic analysis of Phyllanthus species in Thailand and the application of polymerase chain reaction-restriction fragment length polymorphism for Phyllanthus amarus Identifica­tion, Biological & Pharmaceutical Bulletin 33, 1723–1727.

    Article  CAS  Google Scholar 

  40. Srirama, R., Senthilkumar, U., Sreejayan, N., Ravikanth, G., Gurumurthy, B. R., Shivanna, M. B., Sanjappa, M., Ganeshaiah, K. N., and Shaanker, R. U. (2010) Assessing species admixtures in raw drug trade of Phyllanthus, a hepato-protective plant using molecular tools, Journal of Ethnopharmacology 130, 208–215.

    Article  PubMed  CAS  Google Scholar 

  41. Fan, L. L., Zhu, S., Chen, H. B., Yang, D. H., Cai, S. Q., and Komatsu, K. (2009) Identification of the botanical source of Stemonae Radix based on polymerase chain reaction with specific primers and polymerase chain reaction-restriction fragment length polymorphism, Biological & Pharmaceutical Bulletin 32, 1624–1627.

    Article  CAS  Google Scholar 

  42. Vongsak, B., Kengtong, S., Vajrodaya, S., and Sukrong, S. (2008) Sequencing analysis of the medicinal plant Stemona tuberosa and five related species existing in Thailand based on trnH-psbA chloroplast DNA, Planta Medica 74, 1764–1766.

    Article  PubMed  CAS  Google Scholar 

  43. Xue, C. Y., Li, D. Z., Lu, J. M., Yang, J. B., and Liu, J. Q. (2006) Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii, Planta Medica 72, 1223–1226.

    Article  PubMed  CAS  Google Scholar 

  44. Ruzicka, J., Lukas, B., Merza, L., Gohler, I., Abel, G., Popp, M., and Novak, J. (2009) Identification of Verbena officinalis based on ITS sequence analysis and RAPD-derived molecular markers, Planta Medica 75, 1271–1276.

    Article  PubMed  CAS  Google Scholar 

  45. Ronning, S. B., Rudi, K., Berdal, K. G., and Holst-Jensen, A. (2005) Differentiation of important and closely related cereal plant species (Poaceae) in food by hybridization to an oligonucleotide array, Journal of Agricultural and Food Chemistry 53, 8874–8880.

    Article  PubMed  CAS  Google Scholar 

  46. Mafra, I., Ferreira, I., and Oliveira, M. (2008) Food authentication by PCR-based methods, European Food Research and Technology 227, 649–665.

    Article  CAS  Google Scholar 

  47. Uchino, M., Masubuchi, N., Kurosawa, Y., Noguchi, T., and Takano, K. (2007) Specific primer for detection of wheat, Journal of the Japanese Society for Food Science and Technology-Nippon Shokuhin Kagaku Kogaku Kaishi 54, 82–86.

    Article  CAS  Google Scholar 

  48. Shioda, H., Satoh, K., Nagai, F., Okubo, T., Seto, T., Hamano, T., Kamimura, H., and Kano, I. (2003) Identification of Aloe species by random amplified polymorphic DNA (RAPD) analysis, Journal of the Food Hygienic Society of Japan 44, 203–207.

    Article  PubMed  CAS  Google Scholar 

  49. Sasikumar, B., Syamkumar, S., Remya, R., and Zachariah, T. J. (2004) PCR based detection of adulteration in the market samples of ­turmeric powder, Food Biotechnology 18, 299–306.

    Article  CAS  Google Scholar 

  50. Marieschi, M., Torelli, A., Poli, F., Bianchi, A., and Bruni, R. (2010) Quality control of commercial Mediterranean oregano: Development of SCAR markers for the detection of the adulterants Cistus incanus L., Rubus caesius L. and Rhus coriaria L, Food Control 21, 998–1003.

    Article  CAS  Google Scholar 

  51. Meyer, R. (1999) Development and application of DNA analytical methods for the detection of GMOs in food, Food Control 10, 391–399.

    Article  Google Scholar 

  52. White, E., Hunter, J., Dubetz, G., Brost, R., Bratton, A., Edes, S., and Sahota, R. (2000) Microsatellite markers for individual tree genotyping: application in forest crime prosecutions, Journal of Chemical Technology and Biotechnology 75, 923–926.

    Article  CAS  Google Scholar 

  53. Deguilloux, M. F., Pemonge, M. H., and Petit, R. J. (2004) DNA-based control of oak wood geographic origin in the context of the cooperage industry, Annals of Forest Science 61, 97–104.

    Article  CAS  Google Scholar 

  54. Asif, M. J., and Cannon, C. H. (2005) DNA extraction from processed wood: A case study for the identification of an endangered timber species (Gonystylus bancanus), Plant Molecular Biology Reporter 23, 185–192.

    Article  CAS  Google Scholar 

  55. Tnah, L. H., Lee, S. L., Ng, K. K. S., Tani, N., Bhassu, S., and Othman, R. Y. (2009) Geographical traceability of an important tropical timber (Neobalanocarpus heimii) inferred from chloroplast DNA, Forest Ecology and Management 258, 1918–1923.

    Article  Google Scholar 

  56. Chen, F., Chan, H. Y. E., Wong, K. L., Wang, J., Yu, M. T., But, P. P. H., and Shaw, P. C. (2008) Authentication of Saussurea lappa, and endangered medicinal material, by ITS DNA and 5 S rRNA sequencing, Planta Medica 74, 889–892.

    Article  PubMed  CAS  Google Scholar 

  57. Eurlings, M. C. M., van Beek, H. H., and Gravendeel, B. (2010) Polymorphic microsatellites for forensic identification of agarwood (Aquilaria crassna), Forensic Science International 197, 30–34.

    Article  PubMed  CAS  Google Scholar 

  58. Kaundun, S. S., and Matsumoto, S. (2004) PCR-based amplicon length polymorphisms (ALPs) at microsatellite loci and indels from non-coding DNA regions of cloned genes as a means of authenticating commercial Japanese green teas, Journal of the Science of Food and Agriculture 84, 895–902.

    Article  CAS  Google Scholar 

  59. Honjo, M., Ueno, S., Tsumura, Y., Handa, T., Washitani, I., and Ohsawa, R. (2008) Tracing the origins of stocks of the endangered species Primula sieboldii using nuclear microsatellites and chloroplast DNA, Conservation Genetics 9, 1139–1147.

    Article  CAS  Google Scholar 

  60. Tnah, L. H., Lee, S. L., Ng, K. K. S., Faridah, Q. Z., and Faridah-Hanum, I. (2010) Forensic DNA profiling of tropical timber species in Peninsular Malaysia, Forest Ecology and Management 259, 1436–1446.

    Article  Google Scholar 

  61. Bandelj, D., Jakse, J., and Javornik, B. (2002) DNA fingerprinting of olive varieties by microsatellite markers, Food Technology and Biotechnology 40, 185–190.

    CAS  Google Scholar 

  62. Pasqualone, A., Montemurro, C., Summo, C., Sabetta, W., Caponio, F., and Blanco, A. (2007) Effectiveness of microsatellite DNA markers in checking the identity of protected designation of origin extra virgin olive oil, Journal of Agricultural and Food Chemistry 55, 3857–3862.

    Article  PubMed  CAS  Google Scholar 

  63. Brunings, A. M., Moyer, C., Peres, N., and Folta, K. M. (2010) Implementation of simple sequence repeat markers to genotype Florida strawberry varieties, Euphytica 173, 63–75.

    Article  CAS  Google Scholar 

  64. Thomas, M. R., Cain, P., and Scott, N. S. (1994) DNA typing of grapevines: A universal methodology and database for describing cultivars and evaluating genetic relatedness, Plant Molecular Biology 25, 939–949.

    Article  PubMed  CAS  Google Scholar 

  65. Congiu, L., Chicca, M., Cella, R., Rossi, R., and Bernacchia, G. (2000) The use of random amplified polymorphic DNA (RAPD) markers to identify strawberry varieties: a forensic application, Molecular Ecology 9, 229–232.

    Article  PubMed  CAS  Google Scholar 

  66. Pafundo, S., Agrimonti, C., Maestri, E., and Marmiroli, N. (2007) Applicability of SCAR markers to food genomics: Olive oil traceability, Journal of Agricultural and Food Chemistry 55, 6052–6059.

    Article  Google Scholar 

  67. Barthelson, R. A., Sundareshan, P., Galbraith, D. W., and Woosley, R. L. (2006) Development of a comprehensive detection method for medicinal and toxic plant species, American Journal of Botany 93, 566–574.

    Article  PubMed  CAS  Google Scholar 

  68. Miller Coyle, H., Palmbach, T., Juliano, N., Ladd, C., and Lee, H. C. (2003) An overview of DNA methods for the identification and individualization of marijuana, Croatian Medical Journal 44, 315–321.

    PubMed  Google Scholar 

  69. Gigliano, G. S. (1998) Identification of Cannabis sativa L. (Cannabaceae) using restriction profiles of the Internal Transcribed Spacer II (ITS2), Science & Justice 38, 225–230.

    Article  CAS  Google Scholar 

  70. Linacre, A., and Thorpe, J. (1998) Detection and identification of cannabis by DNA, Forensic Science International 91, 71–76.

    Article  PubMed  CAS  Google Scholar 

  71. Miller Coyle, H., Shutler, G., Abrams, S., Hanniman, J., Neylon, S., Ladd, C., Palmbach, T., and Lee, H. C. (2003) A simple DNA extraction method for marijuana samples used in amplified fragment length polymorphism (AFLP) analysis, Journal of Forensic Sciences 48, 343–347.

    PubMed  Google Scholar 

  72. Pinarkara, E., Kayis, S. A., Hakki, E. E., and Sag, A. (2009) RAPD analysis of seized marijuana (Cannabis sativa L.) in Turkey, Electronic Journal of Biotechnology 12.

    Google Scholar 

  73. Gilmore, S., Peakall, R., and Robertson, J. (2003) Short tandem repeat (STR) DNA markers are hypervariable and informative in Cannabis sativa: implications for forensic investigations, Forensic Science International 131, 65–74.

    Article  PubMed  CAS  Google Scholar 

  74. Gilmore, S., Peakall, R., and Robertson, J. (2007) Organelle DNA haplotypes reflect crop-use characteristics and geographic origins of Cannabis sativa, Forensic Science International 172, 179–190.

    Article  PubMed  CAS  Google Scholar 

  75. Howard, C., Gilmore, S., Robertson, J., and Peakall, R. (2009) A Cannabis sativa STR genotype database for Australian seizures: Forensic applications and limitations, Journal of Forensic Sciences 54, 556–563.

    Article  PubMed  CAS  Google Scholar 

  76. Sukrong, S., Zhu, S., Ruangrungsi, N., Phadungcharoen, T., Palanuvej, C., and Komatsu, K. (2007) Molecular analysis of the genus Mitragyna existing in Thailand based on rDNA ITS sequences and its application to identify a narcotic species: Mitragyna speciosa, Biological & Pharmaceutical Bulletin 30, 1284–1288.

    Article  CAS  Google Scholar 

  77. Maruyama, T., Kawamura, M., Kikura-Hanajiri, R., Takayama, H., and Goda, Y. (2009) The botanical origin of kratom (Mitragyna speciosa; Rubiaceae) available as abused drugs in the Japanese markets, Journal of Natural Medicines 63, 340–344.

    Article  PubMed  CAS  Google Scholar 

  78. Schuelke, M. (2000) An economic method for the fluorescent labeling of PCR fragments, Nature Biotechnology 18, 233–234.

    Article  PubMed  CAS  Google Scholar 

  79. Glenn, T. C., and Schable, N. A. (2005) Isolating microsatellite DNA loci, In Molecular Evolution: Producing the Biochemical Data, Part B, pp 202–222.

    Google Scholar 

  80. Raymond, M., and Rousset, F. (1995) GENEPOP (version 1.2): Population genetics software for exact tests and ecumenicism, Journal of Heredity 86, 248–249.

    Google Scholar 

  81. Rousset, F. (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux, Molecular Ecology Resources 8, 103–106.

    Article  PubMed  Google Scholar 

  82. Peakall, R., and Smouse, P. E. (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research, Molecular Ecology Notes 6, 288–295.

    Google Scholar 

  83. Paetkau, D., Slade, R., Burden, M., and Estoup, A. (2004) Genetic assignment methods for the direct, real-time estimation of migration rate: a simulation-based exploration of accuracy and power, Molecular Ecology 13, 55–65.

    Article  PubMed  CAS  Google Scholar 

  84. Pritchard, J. K., Stephens, M., Rosenberg, N. A., and Donnelly, P. (2000) Association mapping in structured populations, American Journal of Human Genetics 67, 170–181.

    Article  PubMed  CAS  Google Scholar 

  85. Radstrom, P., Knutsson, R., Wolffs, P., Lovenklev, M., and Lofstrom, C. (2004) Pre-PCR processing - Strategies to generate PCR-compatible samples, Molecular Biotechnology 26, 133–146.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL, USA

    David N. Zaya & Mary V. Ashley

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  1. David N. Zaya
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  2. Mary V. Ashley
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Correspondence to Mary V. Ashley .

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Editors and Affiliations

  1. , Department of S.T.E.M., Roxbury Community College, Columbus Avenue 1234, Roxbury Crossing, 02120, Massachusetts, USA

    Nikolaus J. Sucher

  2. Centre for Complementary Medicine, Research, University of Western Sydney, Narellan Road, Campbelltown, 2560, New South Wales, Australia

    James R. Hennell

  3. , Department of Natural Sciences, Northern Essex Community College, Elliot Street 110, Haverhill, 01830, Massachusetts, USA

    Maria C. Carles

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Zaya, D.N., Ashley, M.V. (2012). Plant Genetics for Forensic Applications. In: Sucher, N., Hennell, J., Carles, M. (eds) Plant DNA Fingerprinting and Barcoding. Methods in Molecular Biology, vol 862. Humana Press. https://doi.org/10.1007/978-1-61779-609-8_4

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  • DOI: https://doi.org/10.1007/978-1-61779-609-8_4

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