Abstract
HLA typing by sequence-specific primers (PCR-SSP) is a commonly used technique in HLA typing in which multiple pairs of cis-located allele-specific primers are used to determine the alleles present in a given DNA sample. Although the technique is around two decades old, it still offers a relatively straight forward way of typing and has the benefit of using commonly available laboratory equipment. Here we describe the background of the PCR-SSP, how to design and validate reactions, and common problems that arise.
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Bunce, M., Passey, B. (2013). HLA Typing by Sequence-Specific Primers. In: Zachary, A., Leffell, M. (eds) Transplantation Immunology. Methods in Molecular Biology, vol 1034. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-493-7_8
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DOI: https://doi.org/10.1007/978-1-62703-493-7_8
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-492-0
Online ISBN: 978-1-62703-493-7
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