Abstract
The design of tissue culture conditions that faithfully reproduce the characteristics of cells in their native environment remains one of the main challenges of cancer stem cell (CSC) biology. Here we describe a detailed methodology for the isolation and expansion of both human colon CSCs and mouse intestinal adenoma together with a brief differentiation and coculture method that proved to be valuable to study the concept of CSCs plasticity.
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Prasetyanti, P.R., Zimberlin, C., De Sousa E. Melo, F., Medema, J.P. (2013). Isolation and Propagation of Colon Cancer Stem Cells. In: Turksen, K. (eds) Stem Cell Niche. Methods in Molecular Biology, vol 1035. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-508-8_21
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DOI: https://doi.org/10.1007/978-1-62703-508-8_21
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-507-1
Online ISBN: 978-1-62703-508-8
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