Skip to main content
SpringerLink
Log in

Hyperbaric oxygen for experimental intracerebral hemorrhage

  • Conference paper
Cerebral Hemorrhage

Part of the book series: Acta Neurochirurgica Supplementum ((NEUROCHIRURGICA,volume 105))

Abstract

Acute brain edema formation contributes to brain injury after intracerebral hemorrhage (ICH). It has been reported that hyperbaric oxygen (HBO) is neuroprotective in cerebral ischemia, subarachnoid hemorrhage, and brain trauma. In this study, we investigated the effects of HBO on brain edema following ICH in rats.

Male Sprague-Dawley rats received intracerebral infusion of autolo-gous whole blood, thrombin, or ferrous iron. HBO (100% O2, 3.0 ATA for 1 h) was initiated 1 h after intracerebral injection. Control rats were exposed to air at room pressure. Brains were sampled at 24 or 72 h for water content, ion measurement, and Western blot analysis. We found that 1 session of HBO reduced perihematomal brain edema (p < 0.05) 24h after ICH. HBO also reduced heat shock protein-32 (HSP-32) levels (p < 0.05) in ipsilateral basal ganglia 24 h after ICH. However, HBO failed to attenuate thrombin-induced brain edema and exaggerated ferrous iron-induced brain edema (p < 0.05). Three sessions of HBO also failed to reduce brain edema 72 h after ICH.

In summary, HBO reduced early perihematomal brain edema and HSP-32 levels in brain. HBO-related brain protection does not occur through reduction in thrombin toxicity because HBO failed to attenuate thrombin-induced brain edema. Our results also indicate that HBO treatment after hematoma lysis for ICH may be harmful, since HBO amplifies iron-induced brain edema.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  1. Carhuapoma JR, Wang PY, Beauchamp NJ, Keyl PM, Hanley DF, Barker PB (2000) Diffusion-weighted MRI and proton MR spec-troscopic imaging in the study of secondary neuronal injury after intracerebral hemorrhage. Stroke 31: 726–732

    PubMed  CAS  Google Scholar 

  2. Henninger N, Kuppers-Tiedt L, Sicard KM, Gunther A, Schneider D, Schwab S (2006) Neuroprotective effect of hyperbaric oxygen therapy monitored by MR-imaging after embolic stroke in rats. Exp Neurol 201: 316–323

    Article  PubMed  CAS  Google Scholar 

  3. Hua Y, Keep RF, Hoff JT, Xi G (2007) Brain injury after intracerebral hemorrhage: the role of thrombin and iron. Stroke 38(2 Suppl): 759–762

    Article  PubMed  CAS  Google Scholar 

  4. Huang FP, Xi G, Keep RF, Hua Y, Nemoianu A, Hoff JT (2002) Brain edema after experimental intracerebral hemorrhage: role of hemoglobin degradation products. J Neurosurg 96: 287–293

    PubMed  Google Scholar 

  5. Lee KR, Colon GP, Betz AL, Keep RF, Kim S, Hoff JT (1996) Edema from intracerebral hemorrhage: the role of thrombin. J Neurosurg 84: 91–96

    Article  PubMed  CAS  Google Scholar 

  6. Lou M, Eschenfelder CC, Herdegen T, Brecht S, Deuschl G (2004) Therapeutic window for use of hyperbaric oxygenation in focal transient ischemia in rats. Stroke 35: 578–583

    Article  PubMed  Google Scholar 

  7. Mayer SA, Lignelli A, Fink ME, Kessler DB, Thomas CE, Swarup R, Van Heertum RL (1998) Perilesional blood flow and edema formation in acute intracerebral hemorrhage: a SPECT study. Stroke 29: 1791–1798

    PubMed  CAS  Google Scholar 

  8. Mendelow AD, Bullock R, Teasdale GM, Graham DI, McCulloch J (1984) Intracranial haemorrhage induced at arterial pressure in the rat. Part 2: Short term changes in local cerebral blood flow measured by autoradiography. Neurol Res 6: 189–193

    PubMed  CAS  Google Scholar 

  9. Mink RB, Dutka AJ (1995) Hyperbaric oxygen after global cerebral ischemia in rabbits reduces brain vascular permeability and blood flow. Stroke 26: 2307–2312

    PubMed  CAS  Google Scholar 

  10. Nakamura T, Keep RF, Hua Y, Schallert T, Hoff JT, Xi G (2004) Deferoxamine-induced attenuation of brain edema and neurological deficits in a rat model of intracerebral hemorrhage. J Neurosurg 100: 672–678

    PubMed  CAS  Google Scholar 

  11. Nath FP, Kelly PT, Jenkins A, Mendelow AD, Graham DI, Teasdale GM (1987) Effects of experimental intracerebral hemorrhage on blood flow, capillary permeability, and histochemistry. J Neurosurg 66: 555–562

    PubMed  CAS  Google Scholar 

  12. Qin Z, Karabiyikoglu M, Hua Y, Silbergleit R, He Y, Keep RF, Xi G (2007) Hyperbaric oxygen-induced attenuation of hemorrhagic transformation after experimental focal transient cerebral ischemia. Stroke 38: 1362–1367

    Article  PubMed  CAS  Google Scholar 

  13. Qureshi AI, Ling GS, Khan J, Suri MF, Miskolczi L, Guterman LR, Hopkins LN (2001) Quantitative analysis of injured, necrotic, and apoptotic cells in a new experimental model of intracerebral hemorrhage. Crit Care Med 29: 152–157

    Article  PubMed  CAS  Google Scholar 

  14. Rockswold SB, Rockswold GL, Defillo A (2007) Hyperbaric oxygen in traumatic brain injury. Neurol Res 29: 162–172

    Article  PubMed  Google Scholar 

  15. Sharp FR, Bernaudin M (2004) HIF1 and oxygen sensing in the brain. Nat Rev Neurosci 5: 437–448

    Article  PubMed  CAS  Google Scholar 

  16. Sheffield PJ (1998) Measuring tissue oxygen tension: a review. Undersea Hyperb Med 25: 179–188

    PubMed  CAS  Google Scholar 

  17. Singhal AB (2007) A review of oxygen therapy in ischemic stroke. Neurol Res 29: 173–183

    Article  PubMed  Google Scholar 

  18. Tanaka A, Yoshinaga S, Nakayama Y, Kimura M, Tomonaga M (1996) Cerebral blood flow and clinical outcome in patients with thalamic hemorrhages: a comparison with putaminal hemorrhages. J Neurol Sci 144: 191–197

    Article  PubMed  CAS  Google Scholar 

  19. Wagner KR, Hua Y, de Courten-Myers GM, Broderick JP, Nishimura RN, Lu SY, Dwyer BE (2000) Tin-mesoporphyrin, a potent heme oxygenase inhibitor, for treatment of intracerebral hemorrhage: in vivo and in vitro studies. Cell Mol Biol (Noisy-le-grand) 46: 597–608

    CAS  Google Scholar 

  20. Wu J, Hua Y, Keep RF, Schallert T, Hoff JT, Xi G (2002) Oxidative brain injury from extravasated erythrocytes after intracerebral hemorrhage. Brain Res 953: 45–52

    Article  PubMed  CAS  Google Scholar 

  21. Wu J, Hua Y, Keep RF, Nakamura T, Hoff JT, Xi G (2003) Iron and iron-handling proteins in the brain after intracerebral hemorrhage. Stroke. 34: 2964–2969

    Article  PubMed  CAS  Google Scholar 

  22. Xi G, Keep RF, Hoff JT (1998) Erythrocytes and delayed brain edema formation following intracerebral hemorrhage in rats. J Neurosurg 89: 991–996

    PubMed  CAS  Google Scholar 

  23. Xi G, Wagner KR, Keep RF, Hua Y, de Courten-Myers GM, Broderick JP, Brott TG, Hoff JT (1998) The role of blood clot formation on early edema development following experimental intracerebral hemorrhage. Stroke 29: 2580–2586

    PubMed  CAS  Google Scholar 

  24. Xi G, Keep RF, Hua Y, Xiang J, Hoff JT (1999) Attenuation of thrombin-induced brain edema by cerebral thrombin preconditioning. Stroke 30: 1247–1255

    PubMed  CAS  Google Scholar 

  25. Xi G, Hua Y, Bhasin RR, Ennis SR, Keep RF, Hoff JT (2001) Mechanisms of edema formation after intracerebral hemorrhage: effects of extravasated red blood cells on blood flow and blood-brain barrier integrity. Stroke 32: 2932–2938

    Article  PubMed  CAS  Google Scholar 

  26. Xi G, Keep RF, Hoff JT (2002) Pathophysiology of brain edema formation. Neurosurg Clin N Am 13: 371–383

    Article  PubMed  Google Scholar 

  27. Xi G, Fewel ME, Hua Y, Thompson BG Jr, Hoff JT, Keep RF (2004) Intracerebral hemorrhage: pathophysiology and therapy. Neurocrit Care 1: 5–18

    Article  PubMed  Google Scholar 

  28. Xi G, Keep RF, Hoff JT (2006) Mechanisms of brain injury after intracerebral haemorrhage. Lancet Neurol 5: 53–63

    Article  PubMed  Google Scholar 

  29. Yin D, Zhang JH (2005) Delayed and multiple hyperbaric oxygen treatments expand therapeutic window in rat focal cerebral ischemic model. Neurocrit Care 2: 206–211

    Article  PubMed  Google Scholar 

  30. Zazulia AR, Diringer MN, Videen TO, Adams RE, Yundt K, Aiyagari V, Grubb RL Jr, Powers WJ (2001) Hypoperfusion without ischemia surrounding acute intracerebral hemorrhage. J Cereb Blood Flow Metab 21: 804–810

    Article  PubMed  CAS  Google Scholar 

  31. Zhang JH, Lo, T, Mychaskiw G, Colohan A (2005) Mechanisms of hyperbaric oxygen and neuroprotection in stroke. Pathophysiology 12: 63–77

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Neurosurgery, University of Michigan Medical School, 109 Zina Pitcher Place, R5018 Biomedical Science Research Building, Ann Arbor, MI, 48109-2200, USA

    Z. Qin, G. Xi, R. F. Keep, Y. He & Y. Hua MD

  2. Department of Emergency Medicine, University of Michigan Medical School, Ann Arbor, MI, USA

    R. Silbergleit

  3. Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China

    Z. Qin

Authors
  1. Z. Qin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Xi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. F. Keep
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. Silbergleit
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Y. He
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Y. Hua MD
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Neurosurgery, School of Medicine, Huashan Hospital, Fudan University, Shanghai, China

    Liang-Fu Zhou , Xian-Cheng Chen  & Feng-Ping Huang ,  & 

  2. Department of Neurosurgery, University of Michigan, Ann Arbor, MI, USA

    Guohua Xi , Richard F. Keep , Ya Hua  & Karin Muraszko , ,  & 

  3. Department of Neurosurgery, Shanghai Changzheng Hospital, Shanghai, China

    Yi-Cheng Lu

Rights and permissions

Reprints and permissions

Copyright information

© 2008 Springer-Verlag

About this paper

Cite this paper

Qin, Z., Xi, G., Keep, R.F., Silbergleit, R., He, Y., Hua, Y. (2008). Hyperbaric oxygen for experimental intracerebral hemorrhage. In: Zhou, LF., et al. Cerebral Hemorrhage. Acta Neurochirurgica Supplementum, vol 105. Springer, Vienna. https://doi.org/10.1007/978-3-211-09469-3_23

Download citation

  • DOI: https://doi.org/10.1007/978-3-211-09469-3_23

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-09468-6

  • Online ISBN: 978-3-211-09469-3

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics

Search

Navigation