Abstract
Our previous studies found that 17-β estradiol attenuates edema formation after intracerebral hemorrhage (ICH). As brain iron overload occurs after ICH and contributes to ICH-induced brain injury, the present study examined the effects of estrogen on iron-induced brain injury in vivo and in vitro.
There were two sets of experiments in this study. In the first set, male Sprague-Dawley rats were pretreated with 17-β estradiol or vehicle prior to an intracerebral injection of ferrous iron. Ferrous iron was injected into the right caudate and the rats were killed 24 h later for brain edema measurement. In the second set, primary cultured neurons were pretreated with different doses of 17-β estradiol or vehicle for 24 h. The cells were then exposed to ferrous iron for 48 h when culture medium was collected for lactate dehydrogenase measurement. Neuronal death was also assessed by live/dead cell assay.
Estrogen pretreatment reduced brain water content (p < 0.01) 24 h after iron injection. Estrogen also protected against iron-induced cell death in cultured neurons. Estrogen reduces iron-induced brain edema in vivo and neuronal death in vitro suggesting estrogen could be a potential therapeutic agent for ICH.
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Acknowledgments
This study was supported by grants NS-017760, NS-039866, and NS-057539 from the National Institutes of Health (NIH), and 0755717Z and 0840016N from American Heart Asso-ciation (AHA). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH or AHA.
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Gu, Y., Xi, G., Liu, W., Keep, R.F., Hua, Y. (2010). Estrogen Reduces Iron-Mediated Brain Edema and Neuronal Death. In: Czernicki, Z., Baethmann, A., Ito, U., Katayama, Y., Kuroiwa, T., Mendelow, D. (eds) Brain Edema XIV. Acta Neurochirurgica Supplementum, vol 106. Springer, Vienna. https://doi.org/10.1007/978-3-211-98811-4_29
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