Abstract
Neuroglobin (Ngb) is a new member of the family of heme proteins and is specifically expressed in neurons of the central and peripheral nervous systems in all vertebrates. In particular, the retina has a 100-fold higher concentration of Ngb than do other nervous tissues. The role of Ngb in the retina is yet to be clarified. Therefore, to understand the functional role of Ngb in the retina after optic nerve injury (ONI), we used two types of retina, from zebrafish and mice, which have permissible and non-permissible capacity for nerve regeneration after ONI, respectively. After ONI, the Ngb protein in zebrafish was upregulated in the amacrine cells within 3 days, whereas in the mouse retina, Ngb was downregulated in the retinal ganglion cells (RGCs) within 3 days. Zebrafish Ngb (z-Ngb) significantly enhanced neurite outgrowth in retinal explant culture. According to these results, we designed an overexpression experiment with the mouse Ngb (m-Ngb) gene in RGC-5 cells (retinal precursor cells). The excess of m-Ngb actually rescued RGC-5 cells under hypoxic conditions and significantly enhanced neurite outgrowth in cell culture. These data suggest that mammalian Ngb has positive neuroprotective and neuritogenic effects that induce nerve regeneration after ONI.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Brittain T, Skommer J, Raychaudhuri S et al (2010) An antiapoptotic neuroprotective role for neuroglobin. Int J Mol Sci 11:2306–2321
Burmester T, Hankeln T (2009) What is the function of neuroglobin? J Exp Biol 212:1423–1428
Burmester T, Weich B, Reinhardt S et al (2000) A vertebrate globin expressed in the brain. Nature 407:520–523
Kamioka Y, Fujikawa C, Ogai K et al (2013) Functional characterization of fish neuroglobin: zebrafish neuroglobin is highly expressed in amacrine cells after optic nerve injury and can translocate into ZF4 cells. Biochim Biophys Acta 1834:1779–1788
Kato S, Matsukawa T, Koriyama Y et al (2013) A molecular mechanism of optic nerve regeneration in fish: the retinoid signaling pathway. Prog Retin Eye Res 37:13–30
Krishnamoorthy RR, Agarwal P, Prasanna G et al (2001) Characterization of a transformed rat retinal ganglion cell line. Brain Res Mol Brain Res 86:1–12
Lechauve C, Augustin S, Roussel D et al (2013) Neuroglobin involvement in visual pathways through the optic nerve. Biochim Biophys Acta 1834:1772–1778
Li RC, Morris MW, Lee SK et al (2008) Neuroglobin protects PC12 cells against oxidative stress. Brain Res 1190:159–166
Li W, Wu Y, Ren C et al (2011) The activity of recombinant human neuroglobin as an antioxidant and free radical scavenger. Proteins 79:115–125
Rajendram R, Rao NA (2007) Neuroglobin in normal retina and retina from eyes with advanced glaucoma. Br J Ophthalmol 91:663–666
Raychaudhuri S, Skommer J, Henty K et al (2010) Neuroglobin protects nerve cells from apoptosis by inhibiting the intrinsic pathway of cell death. Apoptosis 15:401–411
Schmidt M, Giessl A, Laufs T et al (2003) How does the eye breathe? Evidence for neuroglobin-mediated oxygen supply in the mammalian retina. J Biol Chem 278:1932–1935
Wakasugi K, Kitatsuji C, Morishima I (2005) Possible neuroprotective mechanism of human neuroglobin. Ann N Y Acad Sci 1053:220–230
Acknowledgements
This work was supported by Grants-in-Aid for Scientific Research to K.S. (No. 23618006) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer International Publishing Switzerland
About this paper
Cite this paper
Sugitani, K., Koriyama, Y., Ogai, K., Wakasugi, K., Kato, S. (2016). A Possible Role of Neuroglobin in the Retina After Optic Nerve Injury: A Comparative Study of Zebrafish and Mouse Retina. In: Bowes Rickman, C., LaVail, M., Anderson, R., Grimm, C., Hollyfield, J., Ash, J. (eds) Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology, vol 854. Springer, Cham. https://doi.org/10.1007/978-3-319-17121-0_89
Download citation
DOI: https://doi.org/10.1007/978-3-319-17121-0_89
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-17120-3
Online ISBN: 978-3-319-17121-0
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)