Abstract
We evaluated whether JWH133, a selective cannabinoid type 2 receptor (CB2R) agonist, prevented neurogenic pulmonary edema (NPE) after subarachnoid hemorrhage (SAH) by attenuating inflammation. Adult male rats were assigned to six groups: sham-operated, SAH with vehicle, SAH with JWH133 (0.3, 1.0, or 3.0 mg/kg) treatment 1 h after surgery, and SAH with JWH133 (1.0 mg/kg) at 1 h with a selective CB2R antagonist, SR144528 (3.0 mg/kg). The perforation model of SAH was performed and pulmonary wet-to-dry weight ratio was evaluated 24 and 72 h after surgery. Western blot analyses and immunohistochemistry were evaluated 24 h after surgery. JWH133 (1.0 mg/kg) significantly and most strongly improved lung edema 24 h after SAH. SR144528 administration significantly reversed the effects of JWH133 (1.0 mg/kg). SAH-induced increasing levels of myeloperoxidase (MPO) and decreasing levels of a tight junction (TJ) protein, junctional adhesion molecule (JAM)-A, were ameliorated by JWH133 (1.0 mg/kg) administration 24 h after SAH. Immunohistochemical assessment also confirmed substantial leukocyte infiltration in the outside of vessels in SAH, which were attenuated by JWH133 (1.0 mg/kg) injection. CB2R agonist ameliorated lung permeability by inhibiting leukocyte trafficking and protecting tight junction proteins in the lung of NPE after SAH.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Abbreviations
- NPE:
-
neurogenic pulmonary edema
- SAH:
-
subarachnoid hemorrhage
- CB2R:
-
Cannabinoid type 2 receptor
- MPO:
-
myeloperoxidase
- JAM:
-
junctional adhesion molecule
- EBI:
-
early brain injury
- IP:
-
intraperitoneal administration
- TJs:
-
tight junctions
- PBS:
-
phosphate-buffered saline
- ANOVA:
-
analysis of variance.
References
Altay O, Suzuki H, Hasegawa Y, Caner B, Krafft PR, Fujii M, Tang J, Zhang JH (2012) Isoflurane attenuates blood–brain barrier disruption in ipsilateral hemisphere after subarachnoid hemorrhage in mice. Stroke 43:2513–2516
Brathwaite S, Macdonald RL (2014) Current management of delayed cerebral ischemia: update from results of recent clinical trials. Transl Stroke Res 5:207–226
Bühler D, Schüller K, Plesnila N (2014) Protocol for the induction of subarachnoid hemorrhage in mice by perforation of the circle of Willis with an endovascular filament. Transl Stroke Res 5:653–659
Fujii M, Sherchan P, Krafft PR, Rolland WB, Soejima Y, Zhang JH (2014) Cannabinoid type 2 receptor stimulation attenuates brain edema by reducing cerebral leukocyte infiltration following subarachnoid hemorrhage in rats. J Neurol Sci 342:101–106
Fujii M, Sherchan P, Soejima Y, Hasegawa Y, Flores J, Doycheva D, Zhang JH (2014) Cannabinoid receptor type 2 agonist attenuates apoptosis by activation of phosphorylated CREB-Bcl-2 pathway after subarachnoid hemorrhage in rats. Exp Neurol 261:396–403
Fujii M, Yan J, Rolland WB, Soejima Y, Caner B, Zhang JH (2013) Early brain injury, an evolving frontier in subarachnoid hemorrhage research. Transl Stroke Res 4:432–446
Macmillan CS, Grant IS, Andrews PJ (2002) Pulmonary and cardiac sequelae of subarachnoid haemorrhage: time for active management? Intensive Care Med 28:1012–1023
Nourshargh S, Krombach F, Dejana E (2006) The role of JAM-A and PECAM-1 in modulating leukocyte infiltration in inflamed and ischemic tissues. J Leukoc Biol 80:714–718
Piazza O, Venditto A, Tufano R (2011) Neurogenic pulmonary edema in subarachnoid hemorrhage. Panminerva Med 53:203–2101
Pluta RM, Bacher J, Skopets B, Hoffmann V (2014) A non-human primate model of aneurismal subarachnoid hemorrhage (SAH). Transl Stroke Res 5:681–691
Rassler B, Reissig C, Briest W, Tannapfel A, Zimmer HG (2003) Pulmonary edema and pleural effusion in norepinephrine-stimulated rats–hemodynamic or inflammatory effect? Mol Cell Biochem 250:55–63
Schievink WI, Wijdicks EF, Parisi JE, Piepgras DG, Whisnant JP (1995) Sudden death from aneurysmal subarachnoid hemorrhage. Neurology 45:871–874
Sehba FA (2014) Rat endovascular perforation model. Transl Stroke Res 5:660–668
Soini Y (2011) Claudins in lung diseases. Respir Res 12:70
Steinberg J, Halter J, Schiller H, Gatto L, Carney D, Lee HM, Golub L, Nieman G (2005) Chemically modified tetracycline prevents the development of septic shock and acute respiratory distress syndrome in a clinically applicable porcine model. Shock 24:348–356
Tschöp J, Kasten KR, Nogueiras R, Goetzman HS, Cave CM, England LG, Dattilo J, Lentsch AB, Tschöp MH, Caldwell CC (2009) The cannabinoid receptor 2 is critical for the host response to sepsis. J Immunol 183:499–505
Weir BK (1978) Pulmonary edema following fatal aneurysm rupture. J Neurosurg 49:502–507
Acknowledgments
This study is partially supported by National Institutes of Health grant NS081740 to JHZ.
Conflict of Interest
The authors declare no conflicts of interest.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer International Publishing Switzerland
About this chapter
Cite this chapter
Fujii, M., Sherchan, P., Soejima, Y., Doycheva, D., Zhao, D., Zhang, J.H. (2016). Cannabinoid Receptor Type 2 Agonist Attenuates Acute Neurogenic Pulmonary Edema by Preventing Neutrophil Migration after Subarachnoid Hemorrhage in Rats. In: Applegate, R., Chen, G., Feng, H., Zhang, J. (eds) Brain Edema XVI. Acta Neurochirurgica Supplement, vol 121. Springer, Cham. https://doi.org/10.1007/978-3-319-18497-5_24
Download citation
DOI: https://doi.org/10.1007/978-3-319-18497-5_24
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-18496-8
Online ISBN: 978-3-319-18497-5
eBook Packages: MedicineMedicine (R0)