Abstract
Cytological investigation can, in individual cases, aid significantly in the clarification of forensic questions (Mittal et al. 2010; Murphy 1981; Oehmichen 1984). Locating and identifying cells, assigning cells to particular organs or anatomic locations, as well as isolating cell groups or single cells for further molecular genetic examinations must be considered as an option in certain cases.
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References
Albrecht K, Schultheiss D, Rothämel T, Breitmeier D, Tröger HD (2004) Der Nachweis von Spermaspuren in der forensischen Medizin. Kriminalistik 8/9:552–558
Allery JP, Telmon N, Mieusset R, Rouge BD (2001) Cytological detection of spermatozoa: comparison of three staining methods. J Forensic Sci 46:349–351
Anoruo B, van Oorschot R, Mitchell J, Howells D (2007) Isolating cells from non-sperm cellular mixtures using the PALM microlaser micro dissection system. Forensic Sci Int 173:93–96
Anslinger K, Bayer B, Mack B, Eisenmenger W (2007) Sex-specific fluorescent labelling of cells for laser microdissection and DNA profiling. Int J Legal Med 121:54–56
Balick MJ (1988) Lycopodium spores found in condom dusting agent. Nature 332:591
Balick MJ (1989) Lycopodium spores used in condom manufacture: associated health hazards. Econ Bot 43:373–377
Banaschak S, Sibbing U, Brinkmann B (1997) Identifizierung von Gewebeproben bei Verdacht auf Materialvertauschung. Pathologe 18:385–389
Berkefeld K (1993) Eine Nachweismöglichkeit für Kondombenutzung bei Sexualdelikten. Arch Kriminol 192:37–42
Blackledge RD, Vincenti M (1994) Identification of polydimethylsiloxane lubricant traces from latex condoms in cases of sexual assault. J Forensic Sci Soc 34:245–256
Brinkmann B (2004) Forensische DNA-Analytik. Dtsch Ärztebl 101:A2329–A2335
Brinkmann B, Fechner G, Püschel K (1983) Zur Lungenhistologie bei experimentellem Ertrinken. Z Rechtsmed 89:267–277
Brück S, Thias V, Heidorn F, Gruber C, Kramer N, Evers H, Verhoff MA (2010) Sequenzierung aus einzelnen Epithelzellen. HV1 und HV2-Region der mitochondrialen DNA. Rechtsmedizin 20:25–33
Castella X, Valles J, Cabezuelo Ma, Fernandez R, Artigas A (1992) Fat embolism syndrome and pulmonary microvascular cytology. Chest 101:1710–1711
Cremer U (1994) Zum Nachweis von Präservativrückständen in Vaginalabstrichen. Zbl Rechtsmed 42:425
Di Martino D, Giuffrè G, Staiti N et al (2004) Single sperm cell isolation by laser dissection. Forensic Sci Int 146:S151–S153
Elliot K, Hill DS, Lambert C et al (2003) Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides. Forensic Sci Int 137:28–36
Elliott K, Hill DS, Lambert C, Burroughes TR, Gill P (2003) Use of laser microdissection greatly improves the recovery of DNA from sperm on microscopic slides. Forensic Sci Int 137:28–36
Evers H, Heidorn F, Gruber C, Lasczkowski G, Risse M, Dettmeyer R, Verhoff MA (2009) Investigative strategy for the forensic detection of sperm traces. Forensic Sci Med Pathol 5:182–188
Findlay I, Taylor A, Quirke P et al (1997) DNA-fingerprinting from single cells. Nature 389:555–556
French CEV, Jensen CG, Vintiner SK, Elliot DA, McGlashan SR (2008) A novel histological technique for distinguishing between epithelial cells in forensic casework. Forensic Sci Int 178:1–6
Giampalo C, Schneider V, Kowalski BH, Bellaver LA (1987) The cytologic diagnosis of amniotic fluid embolism: a critical reappraisal. Diagn Cytopathol 3:126–128
Hausmann R, Schellmann B (1994) Forensic value of the Lugol’s staining method: further studies on glycogenated epithelium in the male urinary tract. Int J Legal Med 107:147–151
Hausmann R, Pregler C, Schellmann B (1994) The value of the Lugol’s iodine staining technique for the identification of vaginal epithelial cells. Int J Legal Med 106:298–301
Ito N, Hirota T (1992) Histochemical and cytochemical localisation of blood group antigens. Prog Histochem Cytochem 25:1–85
Janssen W, Bärtschi G (1964) Vitale und supravitale Reaktionen der Alveolarzellen nach protrahiertem Sauerstoffmangel. Dtsch Z Gesamt Gerichtl Med 55:47–60
Jones EL, Leon JA (2004) Lugol’s test re-examined again: buccal cells. J Forensic Sci 49:64–67
Junge A, Dettmeyer R, Madea B (2008) Identification of biological samples in a case of contamination of a cytological slide preparation. J Forensic Sci 53:739–741
Karger B, Meyer E, Knudsen PJT, Brinkmann B (1996) DNA typing of cellular material on perforating bullets. Int J Legal Med 10:177–179
Keil W, Kutschka G, Sachs H (1997) Spuren bei Sexualstraftaten. Zum Nachweis von Kondomrückständen in Vaginal- und Penisabstrichen Kriminalistik 51:439–440
Knudsen PJT (1993) Cytology in ballistics – an experimental investigation of tissue fragments on full metal jacketed bullets using routine cytological techniques. Int J Legal Med 106:15–18
Lee KR, Catalano PM, Ortiz-Giroux S (1986) Cytologic diagnosis of amniotic fluid embolism. Report of a case with a unique cytologic feature and emphasis on the difficulty of eliminating squamous contamination. Acta Cytol 30:177–182
Leo A, Pedal I (2010) Diagnostic approaches to acute transfusion reactions. Forensic Sci Med Pathol 6:135–145
Luke JL (1969) Recovery of intact respiratory epithelium from a cloth pillowcase four days following its utilization as a smothering instrument. J Forensic Sci 14:398–401
Marcus BJ, Collins KA, Harley RA (2005) Ancillary studies in amniotic fluid embolism: a case report and review of the literature. Am J Forensic Med Pathol 26:92–95
Maresch W (1961) Zum Nachweis von Gewebsteilchen an Tatwerkzeugen. Dtsch Z Gesamte Gerichtl Med 51:560–562
Martino D, Giuffre G, Staiti N, Simone A, Le Donne M, Saravo L (2004) Single sperm cell isolation by laser microdissection. Forensic Sci Int 146(Suppl):S151–S153
Maynard P, Allwell K, Roux C, Dawson M, Royds D (2001) A protocol for the forensic analysis of condom and personal lubricants found in sexual assault cases. Forensic Sci Int 124:140–156
Mittal T, Muralidhar Saralaya K, Kuruvilla A, Achary C (2010) Sex determination from buccal mucosa scrapes. Int J Legal Med 123:437–440
Mukoyama H, Seta S (1986) The determination of blood groups in tissue samples. Forensic Science Progress 1, Springer, Berlin, Heidelberg, New York, Tokyo
Murphy GK (1981) Applications of cytology to forensic pathology. Acta Cytol (Baltimore) 25:153–156
Murray C, McAlister C, Elliot K (2007) Identification and isolation of male cells using fluorescence in situ hybridisation and laser dissection, for use in the investigation of sexual assault. Forensic Sci Int Genet 1:247252
Nichols CA, Sens MA (1990) Recovery and evaluation by cytologic techniques of trace material retained on bullets. Am J Forensic Med Pathol 11:17–34
Noda H, Yokota M, Tatsumi S, Sugiyama S (2002) Determination of ABO blood grouping from human oral squamous epithelium by the highly sensitive immunohistochemical staining method EnVision+. J Forensic Sci 47:341–344
Oehmichen M (1984) Fortschritte zytologischer Methoden in der forensischen Pathologie. Pathologe 5:200–203
Oppitz E (1969) A new colouring method for the determination of sperm cells in sexual assault cases. Arch Kriminol 144:145–148
Pachar JV, Cameron JM (1992) Scanning electron microscopy: application in the identification of diatoms in cases of drowning. J Forensic Sci 37:860–866
Paterson SK, Jensen CG, Vintiner SK, McGlashan SR (2006) Immunhistochemical staining as a potential method for the identification of vaginal epithelial cells in forensic casework. J Forensic Sci 51:1138–1143
Pedal I (1987) Blutgruppen-Immunhistochemie. Thieme-Verlag, Stuttgart, New York
Pedal I (1995) Immunhistochemische Blutgruppendiagnostik (ABH, Lewis) in der Rechtsmedizin. In: Bratzke HJ, Schröter A (eds) Immunhistochemie in der Rechtsmedizin. Deutsche Hochschulschriften 1068, Hänsel-Hohenhausen, Egelsbach, Frankfurt, Washington DC, pp 14–21
Pedal I, Freislederer A, Reiter C, Depastas G (1990) Zur Immunhistochemie tödlicher Transfusionszwischenfälle. Pathologe 11:143–147
Randall B (1988) Gylcogenated squamous epithelia cells as a marker of foreign body penetration in sexual assault. J Forensic Sci 33:511–514
Randall B, Riss RE (1985) Penile glycogenated epithelial cells as an indicator of recent vaginal intercourse. Am J Clin Pathol 84:524–526
Rothwell TJ, Harvey KJ (1978) The limitations of the Lugol’s iodine staining technique for the identification of vaginal epithelial cells. J Forensic Sci Soc 18:181–184
Sanders CT, Sanchez N, Ballantyne J, Petersen DA (2006a) Laser microdissection separation of pure spermatozoa. J Forensic Sci 51:748–757
Sanders CT, Sanchez N, Ballantyne J, Peterson DA (2006b) Laser microdissection separation of pure spermatozoa from epithelial cells for short tandem repeat analysis. J Forensic Sci 51:748–757
Schaefer HE (1981) Virus und Respirationstrakt – morphogenetische Aspekte pulmonaler Veränderungen. Verh Dtsch Ges Pathol 65:107–127
Simons JL, Vintiner SK (2011) Effects of histological staining on the analysis of human DANN from archived slides. J Forensic Sci 56. doi:10.1111/j.1556-4029.2010.01595.x
Undritz E, Hegg P (1959) Die morphologisch-hämatologische und cytologische Untersuchung eingetrockneter Blutflecken. Schweiz Med Wschr 89:1088–1091
Undritz E, Hegg P (1960) Die morphologische Untersuchung eingetrockneter Blutflecken. II. Teil: Anwendung an einem Beispiel. Schweiz Med Wschr 90:1223–1230
Vandewoestyne M, van Hoofstat D, van Nieuwerburgh F, Deforce D (2009) Automatic detection of spermatozoa for laser capture microdissection. Int J Legal Med 123:169–175
Wehner F, Stiegler A, Schulz MM, Wehner HD, Martin D (2007) Immunzytochemische Untersuchungen biologischer Spuren an Tatklingen. Arch Krim 219:180–190
Wehner F, Moos NRM, Wehner HD, Martin D, Schulz MM (2008) Immunocytochemical examinations of biological traces on expanding bullets (QD-PEP). Forensic Sci Int 182:66–70
Ziskin D, Moulton R (1948) A comparison of oral and vaginal epithelial smears. J Clin Endocrinol Metab 8:146–165
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Dettmeyer, R.B. (2011). Forensic Cytology. In: Forensic Histopathology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-20659-7_18
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