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Characterization of EBV-Infected Epithelial Cell Lines from Gastric Cancer-Bearing Tissues

  • Chapter
Epstein-Barr Virus and Human Cancer

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 258))

Abstract

The long-term goal of our study is to explore how Epstein-Barr virus (EBV) associates with the pathogenesis of gastric carcinoma. The first goal is to establish EBV-positive epithelial cell lines from EBV-infected gastric carcinoma tissues and to characterize the cell lines and EBV infection in the cells. EBV, a ubiquitous human herpesvirus with oncogenic potential, is predominantly associated with the infection of two target tissues in vivo: (1) B lymphocytes, where the infection is largely nonproductive, and (2) the epithelium, in which virus replication occurs (Rickinson and Kieff 1996). Both target tissues are susceptible to EBV-associated malignant change, leading to tumors of B-cell origin, such as Burkitt’s lym-phoma, or of epithelial-cell origin, such as nasopharyngeal carcinoma (NPC) (Pathmanathan et al. 1995). Recently, EBV has also emerged as an etiologic agent implicated in gastric carcinoma (Min et al. 1991; Shibata et al. 1991; Shibata and Weiss 1992; Tokunaga et al. 1993a,b; Fukayama et al. 1994; Imai et al. 1994; Ohfuji et al. 1996; Iwasaki et al. 1998). EBV has been found in most cases of rare gastric lymphoepithelioma-like carcinomas (Min et al. 1991; Shibata et al. 1991; Ohfuji et al. 1996) and a small but significant proportion of common gastric adenocarcinomas (Shibata and Weiss 1992; Tokunaga et al. 1993a,b). EBV infection was found in approximately 7% of Japanese gastric carcinomas (Tokunaga et al. 1993a,b; Fukayama et al. 1994; Imai et al. 1994). The world distribution of gastric carcinomas with EBV infection is shown from 4% to 18% of total gastric carcinoma in different countries (Tashiro et al. 1998)

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Authors and Affiliations

  1. Department of Biosignaling, School of Life Science, Faculty of Medicine, Tottori University, Yonago, 683-8503, Japan

    T. Sairenji, M. Kanamori, N. Takasaka, X. Gao, M. Murakami, Y. Satoh & Y. Hoshikawa

  2. Central Clinical Laboratory, Teikyo University School of Medicine, Tokyo, 173-0003, Japan

    M. Tajima

  3. Department of Second Surgery, Teikyo University School of Medicine, Tokyo, 173-0003, Japan

    K. Okinaga

  4. Department of Clinical Pathology, Teikyo University School of Medicine, Tokyo, 173-0003, Japan

    Y. Miyazawa

  5. First Department of Pathology, Faculty of Medicine, Tottori University, Yonago, 683-8503, Japan

    H. Ito

  6. Department of Pathology, National Institute of Infectious Diseases, Tokyo, 162-0052, Japan

    T. Sairenji & T. Kurata

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  1. T. Sairenji
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  2. M. Tajima
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Editors and Affiliations

  1. Institute for Genetic Medicine, Department of Tumor Virology, Hokkaido University, N15 W7, Kita-Ku, 060-8638, Sapporo, Japan

    Kenzo Takada

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© 2001 Springer-Verlag Berlin Heidelberg

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Sairenji, T. et al. (2001). Characterization of EBV-Infected Epithelial Cell Lines from Gastric Cancer-Bearing Tissues. In: Takada, K. (eds) Epstein-Barr Virus and Human Cancer. Current Topics in Microbiology and Immunology, vol 258. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56515-1_12

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  • DOI: https://doi.org/10.1007/978-3-642-56515-1_12

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-62568-8

  • Online ISBN: 978-3-642-56515-1

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