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Protective Effect of Hydrogen Gas Therapy After Germinal Matrix Hemorrhage in Neonatal Rats

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Intracerebral Hemorrhage Research

Part of the book series: Acta Neurochirurgica Supplementum ((NEUROCHIRURGICA,volume 111))

Abstract

Background: Germinal matrix hemorrhage (GMH) is a neurological disease of very low birth weight premature infants leading to post-hemorrhagic hydrocephalus, cerebral palsy, and mental retardation. Hydrogen (H2) is a potent antioxidant shown to selectively reverse cytotoxic oxygen-radical injury in the brain. This study investigated the therapeutic effect of hydrogen gas after neonatal GMH injury.

Methods: Neonatal rats underwent stereotaxic infusion of clostridial collagenase into the right germinal matrix brain region. Cognitive function was assessed at 3 weeks, and then sensorimotor function, cerebral, cardiac and splenic growths were measured 1 week thereafter.

Results: Hydrogen gas inhalation markedly suppressed mental retardation and cerebral palsy outcomes in rats at the juvenile developmental stage. The administration of H2 gas, early after neonatal GMH, also normalized the brain atrophy, splenomegaly and cardiac hypertrophy 1 month after injury.

Conclusion: This study supports the role of cytotoxic oxygen-radical injury in early neonatal GMH. Hydrogen gas inhalation is an effective strategy to help protect the infant brain from the post-hemorrhagic consequences of brain atrophy, mental retardation and cerebral palsy. Further studies are necessary to determine the mechanistic basis of these protective effects.

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Conflict of interest statement

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Departments of Physiology, Loma Linda University, School of Medicine, Loma Linda, CA, 92354, USA

    Tim Lekic, Anatol Manaenko, William Rolland, Mathew Peterson, Jiping Tang & John H. Zhang

  2. Department of Human Pathology and Anatomy, Loma Linda University, School of Medicine, Loma Linda, CA, 92354, USA

    Nancy Fathali & John H. Zhang

  3. Department of Neurosurgery, Loma Linda University, School of Medicine, Loma Linda, CA, 92354, USA

    John H. Zhang

  4. Department of Physiology, Loma Linda University, School of Medicine, Risley Hall, Room 223, Loma Linda, CA, 92354, USA

    John H. Zhang

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  1. Tim Lekic
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  2. Anatol Manaenko
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  4. Nancy Fathali
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  7. John H. Zhang
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Correspondence to John H. Zhang .

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  1. , Neurosurgery, Physiology, Loma Linda University, Campus Street 11041, Loma Linda, 92530, USA

    John Zhang

  2. School of Medicine, Dept. Neurosurgery, Loma Linda University, Anderson St. 11234, Loma Linda, 92354, California, USA

    Austin Colohan

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Lekic, T. et al. (2011). Protective Effect of Hydrogen Gas Therapy After Germinal Matrix Hemorrhage in Neonatal Rats. In: Zhang, J., Colohan, A. (eds) Intracerebral Hemorrhage Research. Acta Neurochirurgica Supplementum, vol 111. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0693-8_40

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  • DOI: https://doi.org/10.1007/978-3-7091-0693-8_40

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  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-0692-1

  • Online ISBN: 978-3-7091-0693-8

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